Genetic Diversity and Phylogenetic Study of Leishmania Species in Iran by Multilocus Sequence Typing.

IF 0.9 4区医学 Q4 PARASITOLOGY

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI:10.18502/ijpa.v19i2.15853

Sara Nemati, Homa Hajjaran, Ali Khamesipour, Mohsen Falahati Anbaran, Hamed Mirjalali, Asghar Fazaeli

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Abstract

Background: Leishmaniasis is an important public health parasitic infection, which is endemic in many parts of the world, including Iran. We aimed to investigate genetic diversity and phylogenetic relationship among different Leishmania isolates using multi-locus sequence typing (MLST).

Methods: Totally, 41 isolates collected either from patients referred to Leishmaniasis Diagnostics and Treatment Center at Tehran University of Medical Sciences, Tehran, Iran or from animals during 2019-2021, were subjected to the study. They included L. major and L. tropica from human, L. infantum from canine, and L. turanica from rodents from different endemic foci of Iran analyzed using MLST including gp63, g6pdh, lack, nagt, and hsp70 genes.

Results: A total of 5010 bps was analyzed from each isolate. The three targets, nagt, lack, and g6pdh, generated better topology comparing to the other genes. In the 44 isolates, 22 haplotypes (STs) were identified. Leishmania tropica contained the highest number of haplotypes (n=12) comparing to L. major (n=8), L. infantum (n=1) and L. turanica (n=1). All five genomic loci caused separation of Iranian Leishmania species at the species level, indicating conservation of these genes in the Leishmania parasite.

Conclusion: The highest number of haplotypes belonged to L. tropica, indicating that the genetic diversity of this species is higher than that of L. major. It was further confirmed that the MLST is a suitable method to examine genetic variation of Leishmania parasites with respect to evolutionary and epidemiological studies.

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通过多焦点序列分型研究伊朗利什曼病种的遗传多样性和系统发育。

背景：利什曼病是一种重要的公共卫生寄生虫感染，在包括伊朗在内的世界许多地方流行。我们旨在利用多焦点序列分型（MLST）研究不同利什曼病分离株之间的遗传多样性和系统发育关系：本研究共收集了 41 个分离株，这些分离株或来自转诊至伊朗德黑兰医学科学大学利什曼病诊断和治疗中心的患者，或来自 2019-2021 年期间的动物。研究使用 MLST（包括 gp63、g6pdh、lack、nagt 和 hsp70 基因）对这些样本进行了分析：结果：共分析了每个分离株的 5010 个 bps。与其他基因相比，nagt、lack 和 g6pdh 这三个目标基因产生了更好的拓扑结构。在 44 个分离株中，共鉴定出 22 个单体型（ST）。热带利什曼原虫的单倍型数量最多（12 个），而大叶利什曼原虫（8 个）、婴儿利什曼原虫（1 个）和图兰尼加利什曼原虫（1 个）的单倍型数量最少。所有五个基因组位点都导致了伊朗利什曼病种在物种水平上的分离，表明这些基因在利什曼病寄生虫中的保守性：热带利什曼原虫的单倍型数量最多，表明该种的遗传多样性高于主要利什曼原虫。研究进一步证实，在利什曼原虫的进化和流行病学研究方面，MLST 是研究利什曼原虫遗传变异的一种合适方法。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.