Implementation of green-assessed nanotechnology and quality by design approach for development of optical sensor for determination of tobramycin in ophthalmic formulations and spiked human plasma

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

BMC Chemistry Pub Date : 2024-07-15 DOI:10.1186/s13065-024-01234-y

Christine M. El-Maraghy, Passant M. Medhat, Rania M. Hathout, Miriam F. Ayad, Nermine V. Fares

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Abstract

A fast eco-friendly colorimetric method was developed for the determination of Tobramycin in drug substance, ophthalmic formulations, and spiked human plasma using silver nanoparticles optical sensor. Even though tobramycin is non-UV–visible absorbing, the developed method is based on measuring the absorbance quenching of silver nanoparticles resulting from the interaction with tobramycin. Different factors affecting the absorbance intensity were studied as; silver nanoparticle concentration, pH, buffer type, and reaction time using quality by design approach. Validation of the proposed method was performed according to ICH guidelines and was found to be accurate, precise, and sensitive. The linearity range of tobramycin was 0.35–4.0 μg/mL. The optical sensor was successfully applied for the determination of Tobramycin in ophthalmic formulations and spiked human plasma without pre-treatment. Additionally, the binding between Tobramycin and PVP- capped silver nanoparticles was studied using molecular docking software. The method was assessed and compared to colorimetric reported methods for the green character using Green Analytical Procedure Index (GAPI) and Analytical GREEnness calculator (AGREE) tools and found to be greener.

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采用绿色评估纳米技术和质量设计方法开发用于测定眼科制剂和加标人体血浆中托布霉素含量的光学传感器

利用银纳米颗粒光学传感器建立了一种快速、环保的比色法，用于测定药物、眼科制剂和人体血浆中的妥布霉素。尽管妥布霉素不吸收紫外线，但所开发的方法是基于测量银纳米粒子与妥布霉素相互作用产生的吸光度淬灭。采用质量设计法研究了影响吸光度的不同因素：银纳米粒子浓度、pH 值、缓冲液类型和反应时间。根据 ICH 指南对所提出的方法进行了验证，结果表明该方法准确、精确、灵敏。妥布霉素的线性范围为 0.35-4.0 μg/mL。该光学传感器无需预处理即可成功用于测定眼科制剂和加标人体血浆中的妥布霉素。此外，还使用分子对接软件研究了妥布霉素与 PVP 封端的银纳米粒子之间的结合。使用绿色分析程序指数（GAPI）和分析 GREEnness 计算器（AGREE）工具对该方法的绿色特性进行了评估，并与已报道的比色法进行了比较，发现该方法更环保。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Chemistry Chemistry-General Chemistry

CiteScore

5.30

自引率

2.20%

发文量

审稿时长

27 weeks

期刊介绍： BMC Chemistry, formerly known as Chemistry Central Journal, is now part of the BMC series journals family. Chemistry Central Journal has served the chemistry community as a trusted open access resource for more than 10 years – and we are delighted to announce the next step on its journey. In January 2019 the journal has been renamed BMC Chemistry and now strengthens the BMC series footprint in the physical sciences by publishing quality articles and by pushing the boundaries of open chemistry.