Comparative in-vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Gunica Harjai, Raghavendra Shanbhog
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引用次数: 0

Abstract

Background: Tooth avulsion necessitates swift replantation, for which the preservation of periodontal ligament (PDL) cell viability is paramount. Various storage media have been explored, yet a comparison between amniotic fluid (AF) obtained at different gestational stages (amniocentesis and full-term) and HBSS is lacking.

Aim: This study aims to evaluate AF (amniocentesis and full-term) against HBSS in sustaining PDL cell viability and regulating apoptosis at different time points.

Material and methods: Periodontal fibroblasts cultured in α-MEM were treated with 100% AF (amniocentesis), 100% AF (full-term), and HBSS, incubated for 1, 3, 24, and 48 h at 37°C, and assessed using the MTT assay for viability and AO/EB staining for apoptosis, which was analyzed via fluorescent microscopy after 24 h. Statistical analysis was conducted using one-way ANOVA, multivariate ANOVA, and post hoc Tukey's multiple comparison tests (p < .05).

Results: Amniotic fluid (amniocentesis) exhibited the highest optical density (OD), which implies the highest cell viability across time intervals, followed by AF (full-term) and HBSS. While HBSS maintained PDL morphology, both AF groups showed altered morphology. No cell death was observed after 24 h.

Conclusions: Within the limitations of this study, both AF groups showed the potential to sustain PDL cell viability after 1, 3, 24, and 48 h of storage. However, further investigation is warranted regarding their suitability as storage media.

羊水与 HBSS 在维持牙周成纤维细胞活力和调节其凋亡方面的体外疗效比较分析。
背景:牙齿脱落后需要迅速重新种植,而牙周韧带(PDL)细胞活力的保存至关重要。目的:本研究旨在评估羊水(羊膜腔穿刺术和足月产)与 HBSS 在不同时间点维持 PDL 细胞活力和调节细胞凋亡的作用:用100%羊水(羊膜腔穿刺液)、100%羊水(足月)和HBSS处理在α-MEM中培养的牙周成纤维细胞,在37°C下培养1、3、24和48小时,用MTT法评估细胞活力,用AO/EB染色法评估细胞凋亡,24小时后用荧光显微镜分析细胞凋亡:羊水(羊膜穿刺术)显示出最高的光密度(OD),这意味着不同时间间隔内细胞存活率最高,其次是羊水(足月)和 HBSS。HBSS 保持了 PDL 的形态,而 AF 两组的形态都发生了改变。24 小时后未观察到细胞死亡:在本研究的限制条件下,AF 组和 HBSS 组都显示出在储存 1、3、24 和 48 小时后维持 PDL 细胞活力的潜力。不过,还需要进一步研究它们作为储存介质的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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