Comparative in-vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS

IF 2.3 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Traumatology Pub Date : 2024-07-15 DOI:10.1111/edt.12980

Gunica Harjai, Raghavendra Shanbhog

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引用次数: 0

Abstract

Background

Tooth avulsion necessitates swift replantation, for which the preservation of periodontal ligament (PDL) cell viability is paramount. Various storage media have been explored, yet a comparison between amniotic fluid (AF) obtained at different gestational stages (amniocentesis and full-term) and HBSS is lacking.

Aim

This study aims to evaluate AF (amniocentesis and full-term) against HBSS in sustaining PDL cell viability and regulating apoptosis at different time points.

Material and Methods

Periodontal fibroblasts cultured in α-MEM were treated with 100% AF (amniocentesis), 100% AF (full-term), and HBSS, incubated for 1, 3, 24, and 48 h at 37°C, and assessed using the MTT assay for viability and AO/EB staining for apoptosis, which was analyzed via fluorescent microscopy after 24 h. Statistical analysis was conducted using one-way ANOVA, multivariate ANOVA, and post hoc Tukey's multiple comparison tests (p < .05).

Results

Amniotic fluid (amniocentesis) exhibited the highest optical density (OD), which implies the highest cell viability across time intervals, followed by AF (full-term) and HBSS. While HBSS maintained PDL morphology, both AF groups showed altered morphology. No cell death was observed after 24 h.

Conclusions

Within the limitations of this study, both AF groups showed the potential to sustain PDL cell viability after 1, 3, 24, and 48 h of storage. However, further investigation is warranted regarding their suitability as storage media.

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羊水与 HBSS 在维持牙周成纤维细胞活力和调节其凋亡方面的体外疗效比较分析。

背景：牙齿脱落后需要迅速重新种植，而牙周韧带（PDL）细胞活力的保存至关重要。目的：本研究旨在评估羊水（羊膜腔穿刺术和足月产）与 HBSS 在不同时间点维持 PDL 细胞活力和调节细胞凋亡的作用：用100%羊水（羊膜腔穿刺液）、100%羊水（足月）和HBSS处理在α-MEM中培养的牙周成纤维细胞，在37°C下培养1、3、24和48小时，用MTT法评估细胞活力，用AO/EB染色法评估细胞凋亡，24小时后用荧光显微镜分析细胞凋亡：羊水（羊膜穿刺术）显示出最高的光密度（OD），这意味着不同时间间隔内细胞存活率最高，其次是羊水（足月）和 HBSS。HBSS 保持了 PDL 的形态，而 AF 两组的形态都发生了改变。24 小时后未观察到细胞死亡：在本研究的限制条件下，AF 组和 HBSS 组都显示出在储存 1、3、24 和 48 小时后维持 PDL 细胞活力的潜力。不过，还需要进一步研究它们作为储存介质的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Traumatology 医学-牙科与口腔外科

CiteScore

6.40

自引率

32.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Dental Traumatology is an international journal that aims to convey scientific and clinical progress in all areas related to adult and pediatric dental traumatology. This includes the following topics: - Epidemiology, Social Aspects, Education, Diagnostics - Esthetics / Prosthetics/ Restorative - Evidence Based Traumatology & Study Design - Oral & Maxillofacial Surgery/Transplant/Implant - Pediatrics and Orthodontics - Prevention and Sports Dentistry - Endodontics and Periodontal Aspects The journal"s aim is to promote communication among clinicians, educators, researchers, and others interested in the field of dental traumatology.