Potential nanomedicinal applications and physicochemical nature of Hyphaene thebaica-reduced nano-samaria.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-12-01 Epub Date: 2024-07-15 DOI:10.1002/jemt.24654
Hamza Elsayed Ahmad Mohamed, Ali Talha Khalil, Khaoula Hkiri, Muhammad Ayaz, Assad Usman, Abdul Sadiq, Farhat Ullah, Muhammad Arif Khan, Ikram Ullah, Malik Maaza
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引用次数: 0

Abstract

Herein we described the biofabrication of samarium oxide nanoparticles (HT-Sm2O3 NPs) by applying the aqueous fruit extract of Hyphaene thebaica was utilized as an eco-friendly chelating agent. The prepared NPs were subjected to various physicochemical properties and potential in biomedical applications. X-ray Diffraction (XRD) pattern revealed sharp peaks that corroborated with the Joint Committee on Powder Diffraction Standards (JCPDS) card no. 00-042-1464. Crystallite size obtained from Debye-Scherrer approximation and Williamson-Hall (W-H) plot was 28.73 and 69.3 nm, respectively. Optical bandgap was calculated by employing Kubelka-Munk (K-M) function and was found to be ~4.58 eV. Raman shift was observed at 121, 351, 424-, and 561 cm-1. Photoluminescence (PL) spectra revealed two major peaks positioned at 360 and 540 nm. The high-resolution transmission electron microscopy (HR-TEM) analysis of HT-Sm2O3 nanoparticles (NPs) showed that they predominantly have spherical to cuboidal shapes. Additionally, the selected area electron diffraction (SAED) pattern presented spotty rings, indicating a high level of crystallinity in these NPs. The potential nanomedicine applications were studied using diverse bioassays using different treatments. The antioxidant activity demonstrated 45.71% ± 1.13% inhibition at 1000 μg/mL. Brine shrimp lethality assay revealed the highest cytotoxicity of 46.67% ± 3.33% at 1000 μg/mL and LC50 value of 1081 μg/mL. HT-Sm2O3 NPs exhibited inhibition of angiogenesis (20.41% ± 1.18%) at of 1000 μg/mL. MTT assay results indicated that HT-Sm2O3 NPs exhibit inhibitory effects on cell lines. Specifically, these NPs showed an IC50 value of 104.6 μg/mL against 3T3 cells. Against MCF-7 cells, the NPs demonstrated an IC50 value of 413.25 μg/mL. Additionally, in the inhibition of acetylcholinesterase (AChE), the newly synthesized NPs showed an IC50 value of 320 μg/mL. The antidiabetic assessment through α-glucosidase and α-amylase inhibition assays revealed, an IC50 value of 380 μg/mL for α-glucosidase and 952 μg/mL for α-amylase was calculated. Overall, our study suggested that the Sm2O3 NPs possess moderate anticancer, cholinesterase inhibition, and antidiabetic potential, however, needs further assessment. RESEARCH HIGHLIGHTS: In this work, nano-samaria is synthesized using an eco-friendly and green approach. The nanoparticles were characterized using techniques such as Raman, HR-TEM, FTIR, DRS, XRD, and so on, and the applications were studied using multiple in vitro bioassays for Diabetes, Alzheimer, and Cancer. The nano-samaria revealed good potential for potential biomedical applications.

hyphaene thebaica-还原纳米沙门氏菌的潜在纳米医药应用和理化性质。
在此,我们介绍了利用白花蛇舌草(Hyphaene thebaica)的水性果实提取物作为生态友好型螯合剂,通过生物制造氧化钐纳米粒子(HT-Sm2O3 NPs)的方法。对制备的 NPs 进行了各种理化性质和生物医学应用潜力的测试。X 射线衍射(XRD)图显示出尖锐的峰值,与粉末衍射标准联合委员会(JCPDS)的卡号 00-042-1464 相吻合。根据德拜-舍勒近似法和威廉森-霍尔(W-H)图得出的晶体尺寸分别为 28.73 纳米和 69.3 纳米。利用 Kubelka-Munk (K-M) 函数计算得出的光带隙为 ~4.58 eV。在 121、351、424- 和 561 cm-1 处观察到拉曼位移。光致发光(PL)光谱显示了位于 360 纳米和 540 纳米的两个主要峰值。HT-Sm2O3 纳米粒子(NPs)的高分辨率透射电子显微镜(HR-TEM)分析表明,它们主要呈球形至立方体形。此外,选区电子衍射(SAED)图案显示出斑点环,表明这些 NPs 具有很高的结晶度。利用不同的生物测定方法和不同的处理方法研究了纳米药物的潜在应用。在 1000 μg/mL 的浓度下,抗氧化活性的抑制率为 45.71% ± 1.13%。盐水虾致死试验显示,在 1000 μg/mL 浓度下,细胞毒性最高,为 46.67% ± 3.33%,半数致死浓度为 1081 μg/mL。HT-Sm2O3 NPs 在 1000 μg/mL 时对血管生成有抑制作用(20.41% ± 1.18%)。MTT 检测结果表明,HT-Sm2O3 NPs 对细胞株有抑制作用。具体而言,这些 NPs 对 3T3 细胞的 IC50 值为 104.6 μg/mL。这些 NPs 对 MCF-7 细胞的 IC50 值为 413.25 μg/mL。此外,在抑制乙酰胆碱酯酶(AChE)方面,新合成的 NPs 的 IC50 值为 320 μg/mL。通过α-葡萄糖苷酶和α-淀粉酶抑制实验进行的抗糖尿病评估显示,α-葡萄糖苷酶的 IC50 值为 380 μg/mL,α-淀粉酶的 IC50 值为 952 μg/mL。总之,我们的研究表明,Sm2O3 NPs 具有适度的抗癌、抑制胆碱酯酶和抗糖尿病潜力,但还需要进一步评估。研究亮点:本研究采用生态友好型绿色方法合成了纳米三聚氰胺。使用拉曼、HR-TEM、傅立叶变换红外光谱、DRS、XRD 等技术对纳米颗粒进行了表征,并使用多种体外生物测定方法研究了其在糖尿病、老年痴呆症和癌症方面的应用。纳米撒玛利亚显示出良好的生物医学应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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