Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi-stage solvent extraction and countercurrent chromatography

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Zhuo Liu, Peng Tian, Luqi Li, Jinxing Guo, Yi Zhou, Shuhui Liu, Zhi Yang
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Abstract

In this study, we employed a combination approach for the preparative separation of constituents from Ginkgo biloba L. leaves. It involved multi-stage solvent extractions utilizing two-phase multi-solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The n-heptane/ethyl acetate/water (1:1:2, v/v) and n-heptane/ethyl acetate/methanol/water (HepEMWat, 7:3:7:3, v/v) solvent systems were screened out as extraction systems. The polarities of the upper and lower phases in the multi-solvent systems were adjustable, enabling the effectively segmented separation of complex constituents in G. biloba L. The segmented products were subsequently directly utilized as samples and separated using CCC with the solvent systems acetate/n-butanol/water (4:1:5, v/v), HepEMWat (5:5:5:5, v/v), and HepEMWat (9:1:9:1, v/v), respectively. As a result, a total of 11 compounds were successfully isolated and identified from a 2 g methanol extract of G. biloba L through two-stage extraction and three CCC separation processes; among them, nine compounds exhibited high-performance liquid chromatography purity exceeding 85%.

结合多级溶剂萃取和逆流色谱法制备分离银杏叶中的成分。
在这项研究中,我们采用了一种组合方法来制备分离银杏叶中的成分。该方法包括利用两相多溶剂系统进行多级溶剂萃取,以及利用三种不同溶剂系统进行逆流色谱分离。筛选出正庚烷/乙酸乙酯/水(1:1:2,v/v)和正庚烷/乙酸乙酯/甲醇/水(HepEMWat,7:3:7:3,v/v)溶剂体系作为萃取体系。然后直接将分段产物作为样品,分别用乙酸乙酯/正丁醇/水(4:1:5, v/v)、HepEMWat(5:5:5:5, v/v)和HepEMWat(9:1:9:1, v/v)溶剂体系进行CCC分离。结果,通过两级萃取和三次 CCC 分离,成功地从 2 克双叶桉叶甲醇提取物中分离鉴定出 11 种化合物,其中 9 种化合物的高效液相色谱纯度超过 85%。
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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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