Lung-resident CD3-NK1.1+CD69+CD103+ Cells Play an Important Role in Bacillus Calmette-Guérin Vaccine-Induced Protective Immunity against Mycobacterium tuberculosis Infection.

IF 3.6 3区 医学 Q2 IMMUNOLOGY
Olamipejo Durojaye, Abhinav Vankayalapati, Padmaja Paidipally, Tanmoy Mukherjee, Ramakrishna Vankayalapati, Rajesh Kumar Radhakrishnan
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引用次数: 0

Abstract

Tissue-resident immune cells play important roles in local tissue homeostasis and infection control. There is no information on the functional role of lung-resident CD3-NK1.1+CD69+CD103+ cells in intranasal Bacillus Calmette-Guérin (BCG)-vaccinated and/or Mycobacterium tuberculosis (Mtb)-infected mice. Therefore, we phenotypically and functionally characterized these cells in mice vaccinated intranasally with BCG. We found that intranasal BCG vaccination increased CD3-NK1.1+ cells with a tissue-resident phenotype (CD69+CD103+) in the lungs during the first 7 d after BCG vaccination. Three months post-BCG vaccination, Mtb infection induced the expansion of CD3-NK1.1+CD69+CD103+ (lung-resident) cells in the lung. Adoptive transfer of lung-resident CD3-NK1.1+CD69+CD103+ cells from the lungs of BCG-vaccinated mice to Mtb-infected naive mice resulted in a lower bacterial burden and reduced inflammation in the lungs. Our findings demonstrated that intranasal BCG vaccination induces the expansion of CD3-NK1.1+CD69+CD103+ (lung-resident) cells to provide protection against Mtb infection.

肺驻留 CD3-NK1.1+CD69+CD103+ 细胞在卡介苗诱导的结核分枝杆菌感染保护性免疫中发挥重要作用
组织驻留免疫细胞在局部组织稳态和感染控制中发挥着重要作用。目前还没有关于肺驻留 CD3-NK1.1+CD69+CD103+ 细胞在接种卡介苗(BCG)和/或结核分枝杆菌(Mtb)感染的小鼠体内的功能作用的信息。因此,我们对接种卡介苗的小鼠体内的这些细胞进行了表型和功能鉴定。我们发现,在接种卡介苗后的头 7 天内,肺部具有组织驻留表型(CD69+CD103+)的 CD3-NK1.1+ 细胞增多。接种卡介苗三个月后,Mtb感染诱导肺部CD3-NK1.1+CD69+CD103+(肺驻留)细胞扩增。将卡介苗接种小鼠肺部的CD3-NK1.1+CD69+CD103+细胞收养性转移至Mtb感染的天真小鼠,可降低细菌负荷并减轻肺部炎症。我们的研究结果表明,鼻内卡介苗接种能诱导CD3-NK1.1+CD69+CD103+(肺驻留)细胞的扩增,从而为Mtb感染提供保护。
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来源期刊
Journal of immunology
Journal of immunology 医学-免疫学
CiteScore
8.20
自引率
2.30%
发文量
495
审稿时长
1 months
期刊介绍: The JI publishes novel, peer-reviewed findings in all areas of experimental immunology, including innate and adaptive immunity, inflammation, host defense, clinical immunology, autoimmunity and more. Special sections include Cutting Edge articles, Brief Reviews and Pillars of Immunology. The JI is published by The American Association of Immunologists (AAI)
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