The short peptide encoded by long non-coding RNA RNF217-AS1 inhibits stomach cancer tumorigenesis, macrophage recruitment, and pro-inflammatory responses.

IF 3 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Amino Acids Pub Date : 2024-07-15 DOI:10.1007/s00726-024-03404-7

Qi Ma, Fei Ma, Bin Zhang, Yonglei Zhang, Liangqun Peng, Xiangnan Li

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Abstract

Certain long non-coding RNAs (lncRNAs) have potential peptide-coding abilities. Here, the role and molecular basis of the RNF217-AS1-encoded peptide in stomach cancer (SC) tumorigenesis were explored. Here, lncRNAs associated with SC pathogenesis and macrophage infiltration and lncRNAs with peptide-coding potential were searched by bioinformatics analysis. The gene mRNA and protein levels were examined by RT-qPCR and western blot assays, respectively. Cell viability, migratory, and invasive abilities were measured by CCK-8, Transwell migration, and Transwell invasion assays, respectively. The potential biological processes related to lncRNA RNF217-AS1 were identified by single-gene GSEA analysis. The effect of RNF217-AS1-encoded peptide on SC tumorigenesis was examined by mouse xenograft experiments. The results showed that lncRNA NR2F1-AS1 and RNF217-AS1 were differentially expressed and associated with macrophage infiltration in SC, and they had the ability to translate into short peptides. The RNF217-AS1 ORF-encoded peptide could reduce SC cell viability, inhibit cell migration and invasion, as well as hinder the development of SC xenograft tumors. The RNF217-AS1 ORF-encoded peptide in human SC AGS cells suppressed THP-1 cell migration, triggered the differential expression of CXCL1/CXCL2/CXCL8/CXCL12, and inactivated the TLR4/NF-κB/STAT1 signaling pathways. As a conclusion, the RNF217-AS1 ORF-encoded peptide hindered SC progression in vitro and in vivo and suppressed macrophage recruitment and pro-inflammatory responses in SC.

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长非编码 RNA RNF217-AS1 编码的短肽可抑制胃癌肿瘤发生、巨噬细胞募集和促炎反应。

某些长非编码 RNA（lncRNA）具有潜在的多肽编码能力。本文探讨了 RNF217-AS1 编码的多肽在胃癌（SC）肿瘤发生中的作用和分子基础。本文通过生物信息学分析，检索了与SC发病机制和巨噬细胞浸润相关的lncRNA和具有多肽编码潜力的lncRNA。分别通过RT-qPCR和Western印迹检测了基因mRNA和蛋白水平。细胞活力、迁移和侵袭能力分别通过 CCK-8、Transwell 迁移和 Transwell 侵袭试验进行测定。通过单基因GSEA分析确定了与lncRNA RNF217-AS1相关的潜在生物学过程。通过小鼠异种移植实验研究了RNF217-AS1编码肽对SC肿瘤发生的影响。结果表明，lncRNA NR2F1-AS1和RNF217-AS1在SC中存在差异表达，并与巨噬细胞浸润有关，它们具有翻译成短肽的能力。RNF217-AS1 ORF编码的多肽能降低SC细胞的活力，抑制细胞迁移和侵袭，阻碍SC异种移植瘤的发展。RNF217-AS1 ORF编码肽在人SC AGS细胞中可抑制THP-1细胞迁移，引发CXCL1/CXCL2/CXCL8/CXCL12的差异表达，并使TLR4/NF-κB/STAT1信号通路失活。总之，RNF217-AS1 ORF编码的多肽能在体外和体内阻碍SC的进展，并抑制SC中巨噬细胞的募集和促炎反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Amino Acids 生物-生化与分子生物学

CiteScore

6.40

自引率

5.70%

发文量

审稿时长

2.2 months

期刊介绍： Amino Acids publishes contributions from all fields of amino acid and protein research: analysis, separation, synthesis, biosynthesis, cross linking amino acids, racemization/enantiomers, modification of amino acids as phosphorylation, methylation, acetylation, glycosylation and nonenzymatic glycosylation, new roles for amino acids in physiology and pathophysiology, biology, amino acid analogues and derivatives, polyamines, radiated amino acids, peptides, stable isotopes and isotopes of amino acids. Applications in medicine, food chemistry, nutrition, gastroenterology, nephrology, neurochemistry, pharmacology, excitatory amino acids are just some of the topics covered. Fields of interest include: Biochemistry, food chemistry, nutrition, neurology, psychiatry, pharmacology, nephrology, gastroenterology, microbiology