A stepwise surface ionization method for ion mobility spectrometry

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2024-07-15 DOI:10.1002/rcm.9862

Jianhua Lin, Xiaoguang Gao, Jian Jia, Xiuli He

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引用次数: 0

Abstract

Rationale

The detection of organic nitrogen compounds in exhaled breath is expected to provide an early warning of diseases such as kidney disease. Detecting these trace disease markers in exhaled breath with complex composition and high moisture content is a challenge. Surface ionization (SI) shows a highly selective ionization of organic nitrogen compounds, and it is a good candidate for breath analysis combined with ion mobility spectrometry (IMS).

Methods

A stepwise SI method of low-temperature adsorption/high-temperature ionization was proposed, and trimethylamine (TMA) was detected when combined with an ion mobility spectrometer. TMA at different concentrations and humidity levels and spiked in human breath was detected to evaluate the method's properties.

Results

TMA with concentrations from 2 to 200 ppb was detected. The peak intensity of the TMA characteristic ions was linearly related to the “e” exponent of the concentration with a curve fit of 0.996. A standard deviation of less than 0.306% was obtained with 10 replicate analyses of 10 ppb TMA. The signal intensity difference between dry and wet (relative humidity > 93%) TMA samples is only 2.7%, and the recovery rate of the sample was 106.819%.

Conclusions

SI-IMS based on the stepwise SI method has the advantages of low ionization temperature, high detection sensitivity, strong resistance to humidity interference, and good repeatability. It is a promising method for detecting organic nitrogen compounds in exhaled breath.

查看原文本刊更多论文

用于离子迁移谱分析的逐步表面电离法。

理由：检测呼出气体中的有机氮化合物有望为肾病等疾病提供早期预警。在成分复杂、水分含量高的呼出气体中检测这些痕量疾病标记物是一项挑战。表面电离（SI）对有机氮化合物具有高度选择性，是结合离子迁移谱（IMS）进行呼气分析的理想方法：方法：提出了一种低温吸附/高温电离的分步 SI 方法，并结合离子迁移谱仪检测了三甲胺（TMA）。检测了不同浓度和湿度水平的三甲胺，并在人体呼气中进行了添加，以评估该方法的特性：结果：检测到的 TMA 浓度为 2 至 200 ppb。TMA 特征离子的峰值强度与浓度的 "e "指数呈线性关系，曲线拟合值为 0.996。对 10 ppb TMA 进行 10 次重复分析后，标准偏差小于 0.306%。干燥和潮湿（相对湿度大于 93%）的 TMA 样品之间的信号强度差仅为 2.7%，样品的回收率为 106.819%：基于分步 SI 法的 SI-IMS 具有电离温度低、检测灵敏度高、抗湿度干扰能力强、重复性好等优点。它是一种检测呼气中有机氮化合物的有效方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.