Increased water intake dilutes protective uromodulin levels in urine and results in increased rates of pyelonephritis in a murine model

IF 5.6 2区 医学 Q1 PHYSIOLOGY
Aimi D. K. Hamilton, Laura V. Sparsoe, Mathias Skov, Nanna Johnsen, Mette H. Chreistensen, Thomas J. Corydon, Helle Praetorius
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Abstract

Aim

Urinary tract infections (UTIs) rank among the most prevalent infections in humans, carrying substantial implications for public health. Women experiencing recurrent UTIs are often advised to boost their fluid intake to help eliminate bacteria. In this study, we explored the impact of elevated fluid consumption during UTIs using a mouse model of pyelonephritis.

Methods

UTI was induced in 8–10 w female BALB/cJ-mice by surgically injecting Escherichia coli (O6:K13:H1) into the bladder whereafter mice were randomized to gel food (GF) or regular chow. Immune response and infection severity were determined 24-h post-infection. In vitro bacterial growth (OD600) was determined in urine from mice or from human volunteers.

Results

Gel feeding increased urine output (1.40 ± 0.77 μL min−1, p < 0.01) and diluted the urine (668.7 ± 177 mOsmol kg−1, p < 0.0001) compared to controls on regular chow (urine output: 0.34 ± 0.27 μL min−1, osmolality: 1439 ± 473.5 mOsmol kg−1). Mice on GF had a higher risk of pyelonephritis (87.5%) and more severe infections (26.22 ± 9.88 CFU mg−1 tissue) compared to controls (43.75%; 3.87 ± 3.56 CFU mg−1, p < 0.01). Correspondingly, the growth of E. coli was markedly reduced at osmolalities above 1200 mOsmol kg−1 compared to 600 mOsmol kg−1 and GF mice had lower urine levels of uromodulin (13.70 ± 1.89 μg mL−1, p < 0.01) compared to controls (24.65 ± 2.70 μg mL−1).

Conclusion

Increased water intake and urine flow in mice will markedly increase the risk of pyelonephritis. The increased risk may reflect reduced urine uromodulin combined with optimized growth conditions for E. coli. The study does not immediately support the notion that established UTIs can be eliminated by increased water intake.

Abstract Image

增加水的摄入量会稀释尿液中的保护性尿调节蛋白水平,导致小鼠肾盂肾炎发病率增加。
目的:尿路感染(UTI)是人类最常见的感染之一,对公共卫生有重大影响。反复发生尿路感染的妇女通常会被建议增加液体摄入量,以帮助消除细菌。在这项研究中,我们利用肾盂肾炎小鼠模型探讨了UTI期间增加液体摄入量的影响:方法:通过手术将大肠杆菌(O6:K13:H1)注入膀胱,诱导 8-10 w 的雌性 BALB/cJ 小鼠发生尿毒症。感染后 24 小时测定免疫反应和感染严重程度。在小鼠或人类志愿者的尿液中测定体外细菌生长(OD600):结果:喂食凝胶可增加尿量(1.40 ± 0.77 μL min-1, p -1, p -1, 渗透压:1439 ± 473.5 mOsmol kg-1)。与对照组(43.75%;3.87 ± 3.56 CFU mg-1,p -1,与 600 mOsmol kg-1 相比)相比,服用 GF 的小鼠患肾盂肾炎的风险更高(87.5%),感染更严重(26.22 ± 9.88 CFU mg-1 组织),而且 GF 小鼠尿液中的尿泌素水平更低(13.70 ± 1.89 μg mL-1,p -1):结论:增加小鼠的水摄入量和尿量会显著增加肾盂肾炎的风险。风险的增加可能反映了尿尿素的减少以及大肠杆菌生长条件的优化。这项研究并不能立即支持通过增加水的摄入量来消除已确诊的尿毒症的观点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Physiologica
Acta Physiologica 医学-生理学
CiteScore
11.80
自引率
15.90%
发文量
182
审稿时长
4-8 weeks
期刊介绍: Acta Physiologica is an important forum for the publication of high quality original research in physiology and related areas by authors from all over the world. Acta Physiologica is a leading journal in human/translational physiology while promoting all aspects of the science of physiology. The journal publishes full length original articles on important new observations as well as reviews and commentaries.
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