Microbial metabolic traits drive the differential contribution of microbial necromass to soil organic carbon between the rhizosphere of absorptive roots and transport roots

Abstract

The rhizosphere is a typical soil microbial hotspot, however, not a homogeneous entity. Due to root functional differentiation, different root functional modules (i.e., absorptive roots and transport roots) can play distinct roles in microbial necromass formation and subsequent soil organic carbon (SOC) sequestration by influencing microbial metabolic activity in the surrounding soil. Yet, how microbial metabolic traits mediated by different root functional modules regulate the accumulation of microbial necromass C (MNC) in the rhizosphere remains poorly understood. Herein, we quantified and compared the differences in the contribution of MNC to SOC between the rhizosphere of two root functional modules, and explored the role of microbial metabolic traits in influencing the contribution of MNC to rhizosphere SOC in different root functional modules in two spruce (Picea asperata Mast.) plantations. Our findings revealed that absorptive roots exhibited a significantly higher contribution of MNC to SOC (32.9-37.5%) compared to transport roots (27.7-30.5%) in the rhizosphere. This suggests that absorptive roots possess a greater ability to promote MNC accumulation in the rhizosphere than transport roots. This observation was mainly attributed to the difference in the trade-offs between microbial growth and investment traits between the two root functional modules. Specifically, the rhizosphere of absorptive roots had greater microbial C use efficiency (CUE), faster growth and turnover rates, lower respiratory quotients and biomass-specific enzyme activity than did those of transport roots, suggesting that absorptive roots support greater microbial growth yields and subsequently greater necromass production. Collectively, our findings demonstrate that the contribution of MNC to SOC in the rhizosphere largely depends on the trade-offs of microbial metabolic traits mediated by root functional differentiation. Our study also provides novel and direct empirical evidence supporting the need to integrate function-based fine root classifications with the different contributions of MNC to SOC sequestration in the rhizosphere into land surface models of C cycling.