Effects of CREB inhibitor in mucoepidermoid carcinoma cell lines

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2024-07-13 DOI:10.1016/j.oooo.2024.04.049

Prof Pablo Agustin Vargas , Dr. Maria Eduarda Pérez-de-Oliveira , Dr. Vivian Petersen Wagner , Prof. Colin David Bingle , Prof. Lynne Bingle

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引用次数: 0

Abstract

Introduction

Mucoepidermoid carcinoma (MEC) is the most common malignant salivary gland tumor and about 50% of cases have the CRTC1-MAML2 translocation. The CREB pathway has been associated with the transforming activity of CRTC1-MAML2. The aim of this study was to determine the effects of CREB inhibition on MECs cells behavior in vitro.

Material and Methods

Two translocation-positive (UM-HMC-2 and H292) and one translocation-negative (H253) MEC cell lines were treated with the CREB-inhibitor, 666.15.

Drug IC50 doses were determined for each cell line. Clonogenic and sphere assays were used to assess survival and percentage of cancer stem cells (CSC) and transwell and scratch assays to evaluate the cells invasive and migratory capacity. Immunofluorescence staining was used to analysed the E-cadherin expression upon CREB-inhibitor administration.

Results

The drug significantly reduced the number of surviving colonies and spheres and invasion, and delayed cell invasion in all cell lines, particularly for UM-HMC-2 cells. The expression of E-cadherin was significant higher in treated UM-HMC-2 and H292 cells.

Conclusions

CREB inhibition efficiently impaired MEC key oncogenic behavior associated with metastasis and drug resistance, such as cell invasion and survival, respectively.

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CREB 抑制剂对粘液表皮样癌细胞系的影响

导言褐上皮样癌（MEC）是最常见的恶性唾液腺肿瘤，约50%的病例有CRTC1-MAML2易位。CREB通路与CRTC1-MAML2的转化活性有关。材料与方法用 CREB 抑制剂 666.15 处理两种易位阳性（UM-HMC-2 和 H292）和一种易位阴性（H253）MEC 细胞系。克隆形成试验和球形试验用于评估存活率和癌症干细胞（CSC）的百分比，跨孔试验和划痕试验用于评估细胞的侵袭和迁移能力。免疫荧光染色用于分析服用 CREB 抑制剂后 E-cadherin 的表达。结论CREB抑制剂可分别有效抑制与转移和耐药性相关的MEC关键致癌行为，如细胞侵袭和存活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.