Platelet-Rich Plasma Promotes the Expansion of Human Myoblasts and Favors the In Vitro Generation of Human Muscle Reserve Cells in a Deeper State of Quiescence.

IF 4.5 3区 医学 Q2 CELL & TISSUE ENGINEERING
Stem Cell Reviews and Reports Pub Date : 2024-10-01 Epub Date: 2024-07-13 DOI:10.1007/s12015-024-10760-0
Axel Tollance, Alexandre Prola, Diego Michel, Axelle Bouche, Antoine Turzi, Didier Hannouche, Sarah Berndt, Thomas Laumonier
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Abstract

Stem cell therapy holds significant potential for skeletal muscle repair, with in vitro-generated human muscle reserve cells (MuRCs) emerging as a source of quiescent myogenic stem cells that can be injected to enhance muscle regeneration. However, the clinical translation of such therapies is hampered by the need for fetal bovine serum (FBS) during the in vitro generation of human MuRCs. This study aimed to determine whether fresh allogeneic human platelet-rich plasma (PRP) combined or not with hyaluronic acid (PRP-HA) could effectively replace xenogeneic FBS for the ex vivo expansion and differentiation of human primary myoblasts. Cells were cultured in media supplemented with either PRP or PRP-HA and their proliferation rate, cytotoxicity and myogenic differentiation potential were compared with those cultured in media supplemented with FBS. The results showed similar proliferation rates among human myoblasts cultured in PRP, PRP-HA or FBS supplemented media, with no cytotoxic effects. Human myoblasts cultured in PRP or PRP-HA showed reduced fusion ability upon differentiation. Nevertheless, we also observed that human MuRCs generated from PRP or PRP-HA myogenic cultures, exhibited increased Pax7 expression and delayed re-entry into the cell cycle upon reactivation, indicating a deeper quiescent state of human MuRCs. These results suggest that allogeneic human PRP effectively replaces FBS for the ex vivo expansion and differentiation of human myoblasts and favors the in vitro generation of Pax7High human MuRCs, with important implications for the advancement of stem cell-based muscle repair strategies.

Abstract Image

富血小板血浆促进人类肌母细胞的扩增并有利于体外生成处于深度衰老状态的人类肌肉储备细胞
干细胞疗法在骨骼肌修复方面具有巨大潜力,体外生成的人类肌肉储备细胞(MuRCs)是一种静止肌源性干细胞来源,可通过注射促进肌肉再生。然而,由于在体外生成人类肌肉储备细胞的过程中需要使用胎牛血清(FBS),这种疗法的临床转化受到了阻碍。本研究旨在确定新鲜异体人血小板丰富血浆(PRP)是否与透明质酸(PRP-HA)结合,是否能有效取代异体 FBS,用于人原发性成肌细胞的体外扩增和分化。在补充了 PRP 或 PRP-HA 的培养基中培养细胞,并将其增殖率、细胞毒性和成肌分化潜能与在补充了 FBS 的培养基中培养的细胞进行比较。结果表明,用 PRP、PRP-HA 或 FBS 补充培养基培养的人肌母细胞增殖率相似,且无细胞毒性作用。用 PRP 或 PRP-HA 培养的人肌母细胞在分化后的融合能力下降。不过,我们也观察到,由 PRP 或 PRP-HA 成肌细胞培养物生成的人 MuRCs 表现出 Pax7 表达增加,重新激活后重新进入细胞周期的时间延迟,这表明人 MuRCs 处于更深的静止状态。这些结果表明,异体人PRP可有效替代FBS,用于人肌母细胞的体内外扩增和分化,并有利于体外生成Pax7高的人MuRCs,这对推进基于干细胞的肌肉修复策略具有重要意义。
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来源期刊
Stem Cell Reviews and Reports
Stem Cell Reviews and Reports 医学-细胞生物学
CiteScore
9.30
自引率
4.20%
发文量
0
审稿时长
3 months
期刊介绍: The purpose of Stem Cell Reviews and Reports is to cover contemporary and emerging areas in stem cell research and regenerative medicine. The journal will consider for publication: i) solicited or unsolicited reviews of topical areas of stem cell biology that highlight, critique and synthesize recent important findings in the field. ii) full length and short reports presenting original experimental work. iii) translational stem cell studies describing results of clinical trials using stem cells as therapeutics. iv) papers focused on diseases of stem cells. v) hypothesis and commentary articles as opinion-based pieces in which authors can propose a new theory, interpretation of a controversial area in stem cell biology, or a stem cell biology question or paradigm. These articles contain more speculation than reviews, but they should be based on solid rationale. vi) protocols as peer-reviewed procedures that provide step-by-step descriptions, outlined in sufficient detail, so that both experts and novices can apply them to their own research. vii) letters to the editor and correspondence. In order to facilitate this exchange of scientific information and exciting novel ideas, the journal has created five thematic sections, focusing on: i) the role of adult stem cells in tissue regeneration; ii) progress in research on induced pluripotent stem cells, embryonic stem cells and mechanism governing embryogenesis and tissue development; iii) the role of microenvironment and extracellular microvesicles in directing the fate of stem cells; iv) mechanisms of stem cell trafficking, stem cell mobilization and homing with special emphasis on hematopoiesis; v) the role of stem cells in aging processes and cancerogenesis.
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