A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Jia Su, Youpin Xu, Mingliang Lei, Yingying Meng, Siqi Zhang, Hongrui Liu, Caicun Zhu, Jinhua Chen, Tianxin Zhang, Jiawei Liu, Yunxiang Lin, Zhaorui Yan, Weitao Li, Jing Wang, Xuewei Chen, Min He
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Abstract

Very-long-chain fatty acids (VLCFAs) regulate biophysical properties of cell membranes to determine growth and development of eukaryotes, such as the pathogenesis of the rice blast fungus Magnaporthe oryzae. The fatty acid elongase Elo1 regulates pathogenesis of M. oryzae by modulating VLCFA biosynthesis. However, it remains unknown whether and how Elo1 associates with other factors to regulate VLCFA biosynthesis in fungal pathogens. Here, we identified Ifa38, Phs1 and Tsc13 as interacting proteins of Elo1 by proximity labelling in M. oryzae. Elo1 associated with Ifa38, Phs1 and Tsc13 on the endoplasmic reticulum (ER) membrane to control VLCFA biosynthesis. Targeted gene deletion mutants Δifa38, Δphs1 and Δtsc13 were all similarly impaired as Δelo1 in vegetative growth, conidial morphology, stress responses in ER, cell wall and membrane. These deletion mutants also displayed severe damage in cell membrane integrity and failed to organize the septin ring that is essential for penetration peg formation and pathogenicity. Our study demonstrates that M. oryzae employs a fatty acid elongase complex to regulate VLCFAs for maintaining or remodelling cell membrane structure, which is important for septin-mediated host penetration.

脂肪酸伸长酶复合物调控细胞膜的完整性以及稻瘟病真菌对隔膜依赖性宿主的感染。
超长链脂肪酸(VLCFA)调节细胞膜的生物物理特性,从而决定真核生物的生长和发育,例如稻瘟病真菌Magnaporthe oryzae的致病机理。脂肪酸伸长酶 Elo1 通过调节 VLCFA 的生物合成来调控 M. oryzae 的致病机理。然而,Elo1 是否以及如何与其他因子结合以调控真菌病原体中的 VLCFA 生物合成仍然未知。在这里,我们通过近距离标记法在 M. oryzae 中鉴定出了 Ifa38、Phs1 和 Tsc13 作为 Elo1 的互作蛋白。Elo1 与内质网(ER)膜上的 Ifa38、Phs1 和 Tsc13 相互作用,控制 VLCFA 的生物合成。靶向基因缺失突变体Δifa38、Δphs1和Δtsc13在无性生殖、分生孢子形态、ER、细胞壁和细胞膜的应激反应方面都与Δelo1有类似的缺陷。这些缺失突变体的细胞膜完整性也受到了严重破坏,无法组织对穿刺钉的形成和致病性至关重要的隔膜环。我们的研究表明,M. oryzae 利用脂肪酸伸长酶复合物来调节 VLCFAs,以维持或重塑细胞膜结构,这对于肽键介导的宿主穿透非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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