Characteristics of multidrug-resistant hypervirulent Klebsiella pneumoniae strains ST29 and K212 harbouring tmexC2-tmexD2-toprJ2

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2024-07-14 DOI:10.1016/j.jgar.2024.06.014

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引用次数: 0

Abstract

Objectives

This study aimed to characterize a tigecycline-resistant hypervirulent Klebsiella pneumoniae (HvKP) strain, identified as KLZT, which carries the tigecycline resistance gene cluster tmexC2-tmexD2-toprJ2 belonging to ST29 and serotype K212.

Methods

Antimicrobial susceptibility and virulence phenotypes were assessed, followed by whole-genome sequencing (WGS) using PacBio II and MiSeq sequencers. Genome annotation was carried out using the RAST server and bioinformatics analysis revealed the genetic characteristics of this strain.

Results

Antimicrobial and virulence phenotype testing indicated that K. pneumoniae strain KLZT could be considered as a multidrug-resistant HvKP. WGS analysis showed that KLZT has a single 5,536,506-bp chromosome containing three plasmids 290,963 bp (pKLZT-1), 199,302 bp (pKLZT-2), and 4820 bp (pKLZT-3) in size, and also includes the ST29 and K212 serotypes. Four (bla_SHV-187, oqxA, oqxB, and fosA6) and six resistance genes (tmexC2-tmxeD2-toprJ2, bla_OXA-1, aac(6′)-Ib-cr, catB3, arr-3, and bla_LEN27) were identified from chromosomal and plasmid pKLZT-1, respectively. Gene-based analysis of the resistance genes of plasmid pKLZT-1 showed that the tigecycline resistance gene cluster-carrying region was flanked by umuC and umuD (umuD-hps-IS5-tmexC2-tmexD2-toprJ2-umuC), as well as other resistance genes and virulence factors (ureB, ureC, and ureG), which were carried by IS5075-Tn3-intI1 -aac(6′)-Ib-cr-bla_OXA-1-catB3-arr-3-bla_LEN27-Tn3-ISkpn26-ureBCG-IS5075.

Conclusions

WGS has revealed that a multidrug-resistant strain, HvKP KLZT, belonging to ST29 with capsular serotype K212, contains a multidrug-resistance plasmid.

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ST29 和 K212 型耐多药高病毒性肺炎克雷伯氏菌携带 tmexC2-tmexD2-toprJ2 的特征。

背景：本研究旨在鉴定一株对替加环素耐药的高病毒性肺炎克雷伯氏菌（HvKP）菌株的特征，该菌株被鉴定为KLZT，携带替加环素耐药基因簇tmexC2-tmexD2-toprJ2，属于ST29和血清型K212：使用 PacBio II 和 MiSeq 测序仪对抗菌药敏感性和毒力表型进行评估，然后进行全基因组测序（WGS）。使用 RAST 服务器进行了基因组注释，生物信息学分析揭示了该菌株的遗传特征：结果：抗菌和毒力表型测试表明，肺炎克雷伯菌株 KLZT 可被视为多重耐药 HvKP。WGS分析表明，该菌株有一条5,536,506bp的染色体，包含3个质粒，大小分别为290963bp（pKLZT-1）、199,302bp（pKLZT-2）和4820bp（pKLZT-3）。KLZT 被分为 ST29 和 K212 血清型。从染色体和质粒pKLZT-1中分别鉴定出5个（blaSHV-187、oqxA、oqxB、fosA6）和6个抗性基因（tmexC2-tmxeD2-toprJ2、blaOXA-1、aac(6')-Ib-cr、catB3、arr-3和blaLEN27）。质粒 pKLZT-1 的抗性基因遗传背景分析表明，替加环素抗性基因簇携带区两侧分别是 umuC 和 umuD（umuD-hps-IS5-tmexC2-tmexD2-toprJ2-umuC）以及其他抗性基因和毒力因子（ureB、IS5075-Tn3-intI1-aac(6')-Ib-cr-blaOXA-1-catB3-arr-3-blaLEN27-Tn3-ISkpn26-ureBCG-IS5075）携带。结论：在此，我们介绍了一种对多种药物耐药的 HvKP KLZT 的 WGS 研究结果，该 HvKP KLZT 属于 ST29，其囊膜血清型 K212 含有一种突变耐药质粒。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.