Engineering two-component systems for advanced biosensing: From architecture to applications in biotechnology

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wenyan Cao, Chao Huang, Xuan Zhou, Shenghu Zhou, Yu Deng
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引用次数: 0

Abstract

Two-component systems (TCSs) are prevalent signaling pathways in bacteria. These systems mediate phosphotransfer between histidine kinase and a response regulator, facilitating responses to diverse physical, chemical, and biological stimuli. Advancements in synthetic and structural biology have repurposed TCSs for applications in monitoring heavy metals, disease-associated biomarkers, and the production of bioproducts. However, the utility of many TCS biosensors is hindered by undesired performance due to the lack of effective engineering methods. Here, we briefly discuss the architectures and regulatory mechanisms of TCSs. We also summarize the recent advancements in TCS engineering by experimental or computational-based methods to fine-tune the biosensor functional parameters, such as response curve and specificity. Engineered TCSs have great potential in the medical, environmental, and biorefinery fields, demonstrating a crucial role in a wide area of biotechnology.

用于先进生物传感的双组分系统工程:从结构到生物技术应用。
双组分系统(TCS)是细菌中普遍存在的信号通路。这些系统介导组氨酸激酶和反应调节器之间的磷酸转移,促进对各种物理、化学和生物刺激的反应。随着合成生物学和结构生物学的发展,TCS 被重新用于监测重金属、疾病相关生物标记物和生产生物产品。然而,由于缺乏有效的工程方法,许多 TCS 生物传感器的实用性受到了不理想性能的阻碍。在此,我们将简要讨论 TCS 的结构和调控机制。我们还总结了通过实验或计算方法微调生物传感器功能参数(如响应曲线和特异性)的 TCS 工程学的最新进展。工程化 TCS 在医疗、环境和生物精炼领域具有巨大潜力,在生物技术的广泛领域发挥着至关重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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