Evaluation of antifungal activity of cerium oxide nanoparticles and associated cellular responses.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-20 DOI:10.1093/bbb/zbae101

Shunsuke Nishino, Sayoko Oiki, Yoshimasa Yamana, Daisuke Hagiwara

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引用次数: 0

Abstract

Cerium oxide (CeO2) nanoparticles, as a metal oxide nanomaterial, are increasingly used for various industrial and biomedical applications. Although their cytotoxicity to bacteria and the associated mechanisms have attracted particular attention, the mechanisms behind their antifungal effects have remained unclear. This study investigated the antifungal properties of CeO2, focusing on Aspergillus oryzae. CeO2 inhibited fungal spore germination on solid substrates, and the effect was fungistatic rather than fungicidal. CeO2 inhibited fungal growth, especially under UV irradiation, and induced reactive oxygen species (ROS) production. Tocopherol reduced the intracellular ROS levels and the growth-inhibitory effects of CeO2, suggesting that ROS are involved in these growth-inhibitory effects. Transcriptomic analysis revealed upregulated expression of genes related to phospholipases and phosphate metabolism. CeO2 affected phosphate ion concentration in the medium, potentially influencing cellular responses. This research provided valuable insights into the antifungal effects of CeO2 application, which differ from those of conventional photocatalysts like TiO2.

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评估氧化铈纳米粒子的抗真菌活性及相关细胞反应。

氧化铈纳米粒子（CeO2）作为一种金属氧化物纳米材料，正越来越多地用于各种工业和生物医学应用。虽然它们对细菌的细胞毒性及其相关机制引起了人们的特别关注，但其抗真菌作用背后的机制仍不清楚。本研究研究了 CeO2 的抗真菌特性，重点是黑曲霉。CeO2 可抑制真菌孢子在固体基质上的萌发，其作用是抑菌而非杀菌。CeO2 可抑制真菌生长，尤其是在紫外线照射下，并诱导活性氧（ROS）的产生。生育酚降低了细胞内 ROS 水平和 CeO2 的生长抑制作用，表明 ROS 参与了这些生长抑制作用。转录组分析显示，与磷脂酶和磷酸盐代谢有关的基因表达上调。CeO2 影响了培养基中磷酸盐离子的浓度，从而可能影响细胞的反应。这项研究为了解 CeO2 应用的抗真菌效果提供了宝贵的见解，它不同于 TiO2 等传统光催化剂的抗真菌效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.