Molecular detection and genotyping of human herpes virus 8 in blood donors in Congo.

Q3 Medicine

Voprosy virusologii Pub Date : 2024-07-05 DOI:10.36233/0507-4088-237

P J Iloukou, A L Boumba, D F Ngombe, N R Massengo, G A Malonga, D Moukassa, M M Ennaji

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引用次数: 0

Abstract

Objectives: Human herpesvirus 8 (HHV8) is rarely studied in Congo, despite its prevalence in Africa. Among healthy individuals, HHV-8 does not always lead to a life-threatening infection; however, in immunocompromised individuals, it could lead to more severe disease. The distribution of HHV-8 genotypes varies depending on ethnicity and geographic region.

Method: A prospective cross-sectional study included 265 samples from healthy blood donors from the National Blood Transfusion Center in Brazzaville, with an average age of 35 years, with extremes ranging from 18 to 60 years. After DNA extraction, a nested PCR was carried out for molecular detection, followed by genotyping by amplification of specific primers.

Result: In this study, 4.9% were positive for molecular detection of HHV-8 DNA. All HHV-8 positive DNA samples that were subjected to genotyping by amplification with specific primers allowing discrimination of two major genotypes (A and B). Genotype A was identified in 5 (1.9%) samples and genotype B in 2 (0.7%) samples, indicating that both genotypes were predominant. The remaining viral DNA samples not identified as the major genotypes were classified as «indeterminate» and consisted of 6 (2.3%) samples.

Conclusion: The results of the study suggest that Congo is an area where HHV-8 infection is endemic.

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刚果献血者中人类疱疹病毒 8 的分子检测和基因分型。

目的：尽管人类疱疹病毒 8 (HHV8) 在非洲很普遍，但刚果却很少对其进行研究。在健康人群中，HHV-8 并不一定会导致危及生命的感染；但在免疫力低下的人群中，它可能会导致更严重的疾病。HHV-8 基因型的分布因种族和地理区域而异：一项前瞻性横断面研究纳入了来自布拉柴维尔国家输血中心的265份健康献血者样本，平均年龄为35岁，极端年龄为18至60岁。提取 DNA 后，进行巢式 PCR 分子检测，然后通过特定引物扩增进行基因分型：结果：在这项研究中，4.9%的患者在 HHV-8 DNA 分子检测中呈阳性。所有呈阳性的 HHV-8 DNA 样本都通过特定引物扩增进行了基因分型，从而区分出两种主要基因型（A 型和 B 型）。其中 5 个样本（1.9%）鉴定出基因 A 型，2 个样本（0.7%）鉴定出基因 B 型，表明这两种基因型都是主要的。其余未确定主要基因型的病毒 DNA 样本被归类为 "不确定"，共有 6 个（2.3%）样本：研究结果表明，刚果是 HHV-8 感染流行的地区。

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来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.