Embedded three-dimensional printing of thick pea-protein-enriched constructs for large, customized structured cell-based meat production.

IF 8.2 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Iris Ianovici, Yedidya Zagury, Noa Afik, Moran Hendel, Neta Lavon, Shulamit Levenberg
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引用次数: 0

Abstract

Recent 3D-printing research showed the potential of using plant-protein-enriched inks to fabricate cultivated meat (CM) via agar-based support baths. However, for fabricating large, customized, structured, thick cellular constructs and further cultivation, improved 3D-printing capabilities and diffusion limit circumvention are warranted. The presented study harnesses advanced printing and thick tissue engineering concepts for such purpose. By improving bath composition and altering printing design and execution, large-scale, marbled, 0.5-cm-thick rib-eye shaped constructs were obtained. The constructs featured stable fibrous architectures comparable to those of structured-meat products. Customized multi-cellular constructs with distinct regions were produced as well. Furthermore, sustainable 1-cm-thick cellular constructs were carefully designed and produced, which successfully maintained cell viability and activity for 3 weeks, through the combined effects of void-incorporation and dynamic culturing. As large, geometrically complex construct fabrication suitable for long-term cellular cultivation was demonstrated, these findings hold great promise for advancing structured CM research.

嵌入式三维打印富含厚豌豆蛋白的构建体,用于大型定制结构化细胞肉类生产。
最近的三维打印研究表明,使用富含植物蛋白的油墨通过基于琼脂的支撑浴制造培养肉(CM)具有潜力。然而,要制造大型、定制化、结构化、厚细胞构建体并进行进一步培养,就必须提高三维打印能力并规避扩散极限。本研究利用先进的打印和厚组织工程(TE)概念实现了这一目的。通过改进浴液成分、改变打印设计和执行,获得了大规模、大理石纹、0.5 厘米厚的肋眼形状的构建体。这些构建物具有稳定的纤维结构,可与结构化肉类产品相媲美。此外,还生产出了具有独特区域的定制多细胞构建体。此外,还精心设计和制作了可持续的 1 厘米厚细胞构建体,通过空隙融入和动态培养的综合作用,成功地保持了细胞活力和活性达 3 周之久。由于大型、几何形状复杂的构造物制造适合长期细胞培养,这些发现为推进结构化中药研究带来了巨大希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biofabrication
Biofabrication ENGINEERING, BIOMEDICAL-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
17.40
自引率
3.30%
发文量
118
审稿时长
2 months
期刊介绍: Biofabrication is dedicated to advancing cutting-edge research on the utilization of cells, proteins, biological materials, and biomaterials as fundamental components for the construction of biological systems and/or therapeutic products. Additionally, it proudly serves as the official journal of the International Society for Biofabrication (ISBF).
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