Selective extraction of carnosic acid, carnosol, and rosmarinic acid from Rosmarinus officinalis L. using supercritical fluid and their antioxidant activity
Meryem Boufetacha , Abdelilah Ayad , Nicolas Thiebault , Nadia Boussetta , Elkhadir Gharibi , Mohammed Benali
{"title":"Selective extraction of carnosic acid, carnosol, and rosmarinic acid from Rosmarinus officinalis L. using supercritical fluid and their antioxidant activity","authors":"Meryem Boufetacha , Abdelilah Ayad , Nicolas Thiebault , Nadia Boussetta , Elkhadir Gharibi , Mohammed Benali","doi":"10.1016/j.supflu.2024.106344","DOIUrl":null,"url":null,"abstract":"<div><p><em>Rosmarinus officinalis</em> L. is one of the most widely exploited medicinal plants for its bioactive compounds, including rosmarinic acid (RA), carnosic acid (CA) and carnosol (CAR). This study aims to selectively extract these compounds, using supercritical fluids extraction with carbon dioxide and ethanol as co-solvents, based on the Hansen solubility parameters approach. This approach predicted that CA and CAR are highly soluble in the supercritical fluid. However, a weak interaction was predicted between RA and this fluid. The experimental findings indicated the highest amounts of RA and CA were observed at 150 bar and 80°C, resulting in yields of 3.43 mg/g dry weight (DW), and 18.9 mg/g DW, respectively. Favourable extraction conditions for CAR were recorded at 250 bar and 40°C, with a content of 19.1 mg/g DW. Evaluation of antioxidant activity using DPPH tests revealed that extracts obtained at 250 bar at 40 and 80°C rich in CAR have a stronger antioxidant power.</p></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Supercritical Fluids","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0896844624001797","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Rosmarinus officinalis L. is one of the most widely exploited medicinal plants for its bioactive compounds, including rosmarinic acid (RA), carnosic acid (CA) and carnosol (CAR). This study aims to selectively extract these compounds, using supercritical fluids extraction with carbon dioxide and ethanol as co-solvents, based on the Hansen solubility parameters approach. This approach predicted that CA and CAR are highly soluble in the supercritical fluid. However, a weak interaction was predicted between RA and this fluid. The experimental findings indicated the highest amounts of RA and CA were observed at 150 bar and 80°C, resulting in yields of 3.43 mg/g dry weight (DW), and 18.9 mg/g DW, respectively. Favourable extraction conditions for CAR were recorded at 250 bar and 40°C, with a content of 19.1 mg/g DW. Evaluation of antioxidant activity using DPPH tests revealed that extracts obtained at 250 bar at 40 and 80°C rich in CAR have a stronger antioxidant power.
期刊介绍:
The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics.
Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.