Circadian transcriptome oscillations in human adipose tissue depend on napping status and link to metabolic and inflammatory pathways.

IF 5.6 2区医学 Q1 Medicine

Sleep Pub Date : 2024-11-08 DOI:10.1093/sleep/zsae160

María Rodríguez-Martín, Fernando Pérez-Sanz, Carolina Zambrano, Juan Luján, Mikael Ryden, Frank A J L Scheer, Marta Garaulet

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引用次数: 0

Abstract

Study objectives: Napping is a common habit in many countries. Nevertheless, studies about the chronic effects of napping on obesity are contradictory, and the molecular link between napping and metabolic alterations has yet to be studied. We aim to identify molecular mechanisms in adipose tissue (AT) that may connect napping and abdominal obesity.

Methods: In this cross-sectional study, we extracted the RNA repeatedly across 24 hours from cultured AT explants and performed RNA sequencing. Circadian rhythms were analyzed using six consecutive time points across 24 hours. We also assessed global gene expression in each group (nappers vs. non-nappers).

Results: With napping, there was an 88% decrease in the number of rhythmic genes compared to that in non-nappers, a reduction in rhythm amplitudes of 29%, and significant phase changes from a coherent unimodal acrophase in non-nappers, towards a scattered and bimodal acrophase in nappers. Those genes that lost rhythmicity with napping were mainly involved in pathways of glucose and lipid metabolism, and of the circadian clock. Additionally, we found differential global gene expression between nappers and non-nappers with 34 genes down- and 32 genes upregulated in nappers. The top upregulated gene (IER3) and top down-regulated pseudogene (VDAC2P2) in nappers have been previously shown to be involved in inflammation.

Conclusions: These new findings have implications for our understanding of napping's relationship with obesity and metabolic disorders.

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人体脂肪组织的昼夜节律转录组振荡取决于午睡状态，并与代谢和炎症途径有关。

研究目的：午睡是许多国家的普遍习惯。然而，关于午睡对肥胖的慢性影响的研究却相互矛盾，午睡与代谢改变之间的分子联系也有待研究。我们的目的是找出可能将午睡与腹部肥胖联系起来的脂肪组织（AT）的分子机制：在这项横断面研究中，我们从培养的脂肪组织外植体中反复提取了 24 小时的 RNA，并进行了 RNA 测序。昼夜节律是通过 24 小时内连续 6 个时间点进行分析的。我们还评估了每组（午睡者与非午睡者）的全局基因表达：结果：在午睡时，88%的基因失去了节律性，这些基因在非午睡者中表现出昼夜节律性，节律振幅降低了29%，相位发生了显著变化，非午睡者的单峰相位连贯，而午睡者的双峰相位分散。那些因午睡而失去节律性的基因主要涉及葡萄糖和脂质代谢途径以及昼夜节律。此外，我们还发现午睡者和非午睡者的全球基因表达存在差异，午睡者有 34 个基因下调，32 个基因上调。拍手者中上调最多的基因（IER3）和下调最多的假基因（VDAC2P2）以前曾被证明与炎症有关：这些新发现可能有助于我们了解午睡对肥胖和代谢紊乱的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sleep Medicine-Neurology (clinical)

CiteScore

8.70

自引率

10.70%

发文量

期刊介绍： SLEEP® publishes findings from studies conducted at any level of analysis, including: Genes Molecules Cells Physiology Neural systems and circuits Behavior and cognition Self-report SLEEP® publishes articles that use a wide variety of scientific approaches and address a broad range of topics. These may include, but are not limited to: Basic and neuroscience studies of sleep and circadian mechanisms In vitro and animal models of sleep, circadian rhythms, and human disorders Pre-clinical human investigations, including the measurement and manipulation of sleep and circadian rhythms Studies in clinical or population samples. These may address factors influencing sleep and circadian rhythms (e.g., development and aging, and social and environmental influences) and relationships between sleep, circadian rhythms, health, and disease Clinical trials, epidemiology studies, implementation, and dissemination research.