In vivo Hyperpolarized Metabolic Imaging to Monitor the Progression of Hepatitis B Virus (HBV)-Related Hepatitis to Liver Fibrosis.

IF 3 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Molecular Imaging and Biology Pub Date : 2024-08-01 Epub Date: 2024-07-11 DOI:10.1007/s11307-024-01936-8

Chung Man Moon, Suk Hee Heo, Yong Yeon Jeong, Yun Young Lee, Seul Kee Kim, Sang Soo Shin

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Abstract

Purpose: This study aimed to assess metabolic changes to monitor the progression from normal liver to hepatitis B virus (HBV)-related hepatitis and liver fibrosis using hyperpolarized ¹³C magnetic resonance imaging (MRI).

Procedures: Hepatitis was induced in mice (n = 16) via hydrodynamic injection of HBV 1.2 plasmid (25 μg). Among them, liver fibrosis was induced in the mice (n = 8) through weight-adapted administration of thioacetamide with ethanol. Normal control mice (n = 8) were injected with a phosphate buffer solution. Subsequently, a hyperpolarized ¹³C MRI was performed on the mouse liver in vivo. The level of hepatitis B surface antigen (HBsAg) in blood serum was measured. Statistical analysis involved comparing the differential metabolite ratios, blood biochemistry values, and body weight among the three groups using the Kruskal-Wallis one-way analysis of variance.

Results: HBsAg was absent in the normal and fibrosis groups, while it was detected in the hepatitis group. The ratios of [1-¹³C] lactate/pyruvate, [1-¹³C] alanine/pyruvate, [1-¹³C] lactate/total carbon, and [1-¹³C] alanine/total carbon were significantly lower in the normal control group than in the hepatitis and fibrosis groups (p < 0.05). Moreover, these ratios were significantly higher in the fibrosis group than in the hepatitis group (p < 0.05). However, no significant differences were observed in either [1-¹³C] pyruvate-hydrate/pyruvate or [1-¹³C] pyruvate-hydrate/total carbon among the three groups.

Conclusions: The levels of [1-¹³C] lactate and [1-¹³C] alanine in vivo may serve as valuable indicators for differentiating between HBV-related hepatitis, liver fibrosis, and normal liver.

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通过体内超极化代谢成像监测乙型肝炎病毒 (HBV) 相关肝炎向肝纤维化的进展。

目的：本研究旨在利用超极化 13C 磁共振成像（MRI）评估代谢变化，以监测从正常肝脏到乙型肝炎病毒（HBV）相关肝炎和肝纤维化的进展：小鼠（n = 16）通过水动力注射 HBV 1.2 质粒（25 μg）诱发肝炎。其中，小鼠（n = 8）的肝纤维化是通过体重适应性给药硫代乙酰胺和乙醇诱发的。正常对照组小鼠（n = 8）注射磷酸盐缓冲溶液。随后，对小鼠肝脏进行了超极化 13C 磁共振成像。血清中乙型肝炎表面抗原（HBsAg）的水平进行了测定。统计分析包括使用 Kruskal-Wallis 单因素方差分析法比较三组之间的差异代谢物比率、血液生化值和体重：结果：正常组和纤维化组均未检出 HBsAg，而肝炎组检出了 HBsAg。正常对照组的[1-13C]乳酸/丙酮酸、[1-13C]丙氨酸/丙酮酸、[1-13C]乳酸/总碳和[1-13C]丙氨酸/总碳的比率显著低于肝炎组和纤维化组（P 13C]丙酮酸水合物/丙酮酸或[1-13C]丙酮酸水合物/总碳在三组中的比率）：体内[1-13C]乳酸和[1-13C]丙氨酸的水平可作为区分 HBV 相关肝炎、肝纤维化和正常肝脏的重要指标。

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来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.