Bat thermoregulation in the heat: seasonal variation in evaporative cooling capacities in four species of European bats

Abstract

Phenotypic flexibility is an important source of physiological variation in endotherms and plays an integral role in species’ response to rapid environmental changes. Studies of phenotypic flexibility have focused on winter acclimatization and cold endurance, and there are fewer data on summer acclimatization and adjustments in heat dissipation capacity, especially in Temperate-Zone species. We used indirect calorimetry and thermometry to test if thermoregulation at high air temperatures (T_a) varies between spring and summer in four species of European vespertilionid bats: Nyctalus noctula, Pipistrellus nathusii, P. pygmaeus, and P. pipistrellus. We measured subcutaneous body temperature (T_sub), evaporative water loss, and resting metabolic rate while exposing bats to a stepped profile of increasing T_a, from 28 °C–48 °C. We predicted that during summer, bats increase heat tolerance and evaporative cooling capacity, to better tolerate hotter T_as. In contrast, we found lower maximum ratios of evaporative heat loss (EHL) to metabolic heat production (MHP) during summer, but no seasonal differences in maximum T_a tolerated or T_sub. The main cause of this seasonal difference in maximum EHL/MHP seems to be from bats increasing EWL more gradually with increasing T_a in summer than spring, particularly in the smaller Pipistrellus species. Therefore, this seasonal variation in heat-dissipation strategies may reflect enhanced water conservation during summer to avoid dehydration, as bats are confined to roosts for longer and hotter days compared to spring.