Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis.

IF 1.1 Q4 VIROLOGY
Advances in Virology Pub Date : 2024-07-04 eCollection Date: 2024-01-01 DOI:10.1155/2024/3263228
Charilaos Dellis, Elizabeth-Barbara Tatsi, Dimitra-Maria Koukou, Filippos Filippatos, Evangelia-Eirini Vetouli, Emmanouil Zoumakis, Athanasios Michos, Vasiliki Syriopoulou
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引用次数: 0

Abstract

Group A rotavirus (RVA), which causes acute gastroenteritis (AGE) in children worldwide, is categorized mainly based on VP7 (genotype G) and VP4 (genotype P) genes. Genotypes that circulate at <1% are considered unusual. Important genes also include VP6 (genotype I) and NSP4 (genotype E). VP6 establishes the group and affects immunogenicity, while NSP4, as an enterotoxin, is responsible for the clinical symptoms. The aim of this study was to genotype the VP6 and NSP4 genes and molecularly characterize the NSP4 and VP6 genes of unusual RVA. Unusual RVA strains extracted from fecal samples of children ≤16 years with AGE were genotyped in VP6 and NSP4 genes with Sanger sequencing. In a 15-year period (2007-2021), 54.8% (34/62) of unusual RVA were successfully I and E genotyped. Three different I and E genotypes were identified; I2 (73.5%, 25/34) and E2 (35.3%, 12/34) were the most common. E3 genotype was detected from 2017 onwards. The uncommon combination of I2-E3 was found in 26.5% (9/34) of the strains and G3-P[9]-I2-E3 remained the most frequent G-P-I-E combination (20.6%, 7/34). Children infected with RVA E2 strains had a statistically higher frequency of dehydration (50%) than those infected with RVA E3 strains (p = 0.019). Multiple substitutions were detected in NSP4, but their functional effect remains unknown. The result indicates the genetic diversity of RVA strains. Continuous surveillance of the RVA based on the whole genome will provide better knowledge of its evolution.

从急性肠胃炎患儿中分离出的 A 组异常轮状病毒 VP6 和 NSP4 基因的基因分型和分子特征。
A 组轮状病毒(RVA)主要根据 VP7(基因型 G)和 VP4(基因型 P)基因进行分类。在 P = 0.019 时流行的基因型)。在 NSP4 中检测到多个置换,但其功能影响仍不清楚。这一结果表明了 RVA 株系的遗传多样性。基于全基因组对 RVA 进行持续监测将有助于更好地了解其进化情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.30
自引率
0.00%
发文量
23
审稿时长
22 weeks
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