Duration of anorexia nervosa is positively associated with whole blood mitochondrial DNA copy number: A cross-sectional case-control study

IF 2.5 4区 医学 Q2 PSYCHIATRY
Eimear Doody , Karen M. Ryan , Clare O'Toole , Declan M. McLoughlin
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引用次数: 0

Abstract

Background and Objectives

Anorexia nervosa (AN) is associated with significant physical and psychological stress. Stress causes changes at a cellular level and mitochondria may reflect this stress via changes such as alterations in DNA quantity or in their function. Mitochondria are unique in that they possess their own DNA, distinct from nuclear DNA, of which there are many copies within the cell. An indirect measure of mitochondrial function is the assessment of mitochondrial DNA copy number (mtDNAcn). Telomere length (TL) represents a marker of normal biological ageing, and TL can be shortened by stressors. We sought to test the following hypotheses: (1) that increased mtDNAcn would be found in patients with AN in comparison to healthy controls, (2) that mtDNAcn is related to duration and severity of AN, and (3) mtDNAcn is correlated with TL.

Methods

Using quantitative real-time polymerase chain reaction, we calculated mtDNAcn and TL using peripheral whole blood DNA collected from patients with AN (n = 23) compared to samples from age- and sex-matched healthy controls (n = 33). Clinical characteristics were also obtained.

Results

We found no difference between mtDNAcn in patients with AN in comparison to controls. mtDNAcn was positively associated with duration of illness, but not with illness severity. There was no association between mtDNAcn and TL.

Conclusions

Further investigations may clarify whether compensatory mechanisms increase mtDNAcn with increased illness duration.

神经性厌食症的持续时间与全血线粒体 DNA 拷贝数呈正相关:横断面病例对照研究
背景和目的神经性厌食症(AN)与巨大的生理和心理压力有关。压力会导致细胞层面的变化,线粒体可能会通过 DNA 数量或功能的变化来反映这种压力。线粒体的独特之处在于它们拥有自己的 DNA,有别于细胞内有许多拷贝的核 DNA。线粒体 DNA 拷贝数(mtDNAcn)的评估是线粒体功能的间接衡量标准。端粒长度(TL)是正常生物衰老的标志,而端粒长度会因压力因素而缩短。我们试图验证以下假设:(1) 与健康对照组相比,AN 患者的 mtDNAcn 会增加;(2) mtDNAcn 与 AN 的持续时间和严重程度有关;(3) mtDNAcn 与端粒长度相关。方法我们使用定量实时聚合酶链式反应,利用从 AN 患者(n = 23)和年龄与性别匹配的健康对照组(n = 33)采集的外周全血 DNA 计算 mtDNAcn 和端粒长度。mtDNAcn与病程呈正相关,但与病情严重程度无关。mtDNAcn与TL之间没有关联。结论进一步的研究可能会澄清代偿机制是否会随着病程的延长而增加mtDNAcn。
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来源期刊
CiteScore
2.90
自引率
0.00%
发文量
40
审稿时长
43 days
期刊介绍: The European journal of psychiatry is a quarterly publication founded in 1986 and directed by Professor Seva until his death in 2004. It was originally intended to report “the scientific activity of European psychiatrists” and “to bring about a greater degree of communication” among them. However, “since scientific knowledge has no geographical or cultural boundaries, is open to contributions from all over the world”. These principles are maintained in the new stage of the journal, now expanded with the help of an American editor.
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