Molecular Detection of Coxiella burnetii in Vaginal Swab Samples from Sheep That Aborted.

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Vector borne and zoonotic diseases Pub Date : 2024-07-11 DOI:10.1089/vbz.2023.0153

Oliver Sánchez-Rodríguez, Erika G Palomares-Resendiz, Efrén Díaz-Aparicio, Beatriz Arellano-Reynoso, Luary C Martínez-Chavarría, Guadalupe Martínez-Serrano, Rigoberto Hernández-Castro

{"title":"Molecular Detection of Coxiella burnetii in Vaginal Swab Samples from Sheep That Aborted.","authors":"Oliver Sánchez-Rodríguez, Erika G Palomares-Resendiz, Efrén Díaz-Aparicio, Beatriz Arellano-Reynoso, Luary C Martínez-Chavarría, Guadalupe Martínez-Serrano, Rigoberto Hernández-Castro","doi":"10.1089/vbz.2023.0153","DOIUrl":null,"url":null,"abstract":"Background: Coxiella burnetii, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. Methods: The objective of this work was to determine the presence of C. burnetii DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and C. burnetii was identified by PCR amplification and sequencing of the IS1111 insertion sequence. Results and Conclusion: In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for C. burnetii. This is the first report of C. burnetii in sheep that aborted and in ram semen in Mexico.","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vector borne and zoonotic diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/vbz.2023.0153","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Coxiella burnetii, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. Methods: The objective of this work was to determine the presence of C. burnetii DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and C. burnetii was identified by PCR amplification and sequencing of the IS1111 insertion sequence. Results and Conclusion: In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for C. burnetii. This is the first report of C. burnetii in sheep that aborted and in ram semen in Mexico.

查看原文本刊更多论文

流产绵羊阴道拭子样本中燃烧性柯西氏菌的分子检测

背景：烧伤柯西氏菌是一种细胞内固有细菌，是人类 Q 热的病原体，也是导致小反刍动物流产的原因之一。尽管柯西氏菌病被认为是一种外来疾病，但在墨西哥却有少数报道。方法：这项工作的目的是确定流产绵羊的阴道样本和公羊精液中是否存在烧伤弧菌 DNA。从墨西哥中部和南部五个州共获得 180 份阴道渗出物样本和 20 份精液样本。从阴道拭子中提取了总 DNA，并通过 PCR 扩增和 IS1111 插入序列测序对烧伤蜱进行了鉴定。结果与结论：共有 110 份（110/180）阴道样本和 12 份（12/20）精液样本对烧伤桿菌呈阳性反应。这是墨西哥首次报告在流产的绵羊和公羊精液中检出烧伤弧菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Vector borne and zoonotic diseases 医学-传染病学

CiteScore

4.70

自引率

4.80%

发文量

审稿时长

3-8 weeks

期刊介绍： Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics. Vector-Borne and Zoonotic Diseases coverage includes: -Ecology -Entomology -Epidemiology -Infectious diseases -Microbiology -Parasitology -Pathology -Public health -Tropical medicine -Wildlife biology -Bacterial, rickettsial, viral, and parasitic zoonoses