Dual-targeting liposomes modified with BTP-7 and pHA for combined delivery of TCPP and TMZ to enhance the anti-tumour effect in glioblastoma cells.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-09-01 Epub Date: 2024-07-11 DOI:10.1080/02652048.2024.2376114

Lili Li, Lin Jing, Zijun Tang, Jingguo Du, Yonglong Zhong, Xu Liu, Mingqing Yuan

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引用次数: 0

Abstract

Aim: To construct a novel nano-carrier with dual ligands to achieve superior anti-tumour efficacy and lower toxic side effects.

Methods: Liposomes were prepared by thin film hydration method. Ultraviolet, high performance liquid chromatography, nano-size analyser, ultrafiltration centrifugation, dialysis, transmission electron microscope, flow cytometry, Cell Counting Kit-8, confocal laser scanning microscopy, transwell, and tumorsphere assay were used to study the characterisations, cytotoxicity, and in vitro targeting of dg-Bcan targeting peptide (BTP-7)/pHA-temozolomide (TMZ)/tetra(4-carboxyphenyl)porphyrin (TCPP)-Lip.

Results: BTP-7/pHA-TMZ/TCPP-Lip was a spheroid with a mean diameters of 143 ± 3.214 nm, a polydispersity index of 0.203 ± 0.025 and a surface charge of -22.8 ± 0.425 mV. The drug loadings (TMZ and TCPP) are 7.40 ± 0.23% and 2.05 ± 0.03% (mg/mg); and the encapsulation efficiencies are 81.43 ± 0.51% and 84.28 ± 1.64% (mg/mg). The results showed that BTP-7/pHA-TMZ/TCPP-Lip presented enhanced targeting and cytotoxicity.

Conclusion: BTP-7/pHA-TMZ/TCPP-Lip can specifically target the tumour cells to achieve efficient drug delivery, and improve the anti-tumour efficacy and reduces the systemic toxicity.

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用 BTP-7 和 pHA 修饰的双靶向脂质体联合递送 TCPP 和 TMZ，以增强对胶质母细胞瘤细胞的抗肿瘤作用。

目的：构建具有双重配体的新型纳米载体，以实现卓越的抗肿瘤疗效和较低的毒副作用：方法：采用薄膜水合法制备脂质体。紫外、高效液相色谱、纳米粒度分析仪、超滤离心、透析、透射电子显微镜、流式细胞仪、细胞计数试剂盒-8、激光共聚焦扫描显微镜、研究了 dg-Bcan 靶向肽（BTP-7）/pHA-替莫唑胺（TMZ）/四（4-羧基苯基）卟啉（TCPP）-Lip 的特性、细胞毒性和体外靶向性。结果BTP-7/pHA-TMZ/TCPP-Lip 为球形，平均直径为 143 ± 3.214 nm，多分散指数为 0.203 ± 0.025，表面电荷为 -22.8 ± 0.425 mV。药物负载量（TMZ 和 TCPP）分别为 7.40 ± 0.23% 和 2.05 ± 0.03%（毫克/毫克）；封装效率分别为 81.43 ± 0.51% 和 84.28 ± 1.64%（毫克/毫克）。结果表明，BTP-7/pHA-TMZ/TCPP-Lip 具有更强的靶向性和细胞毒性：结论：BTP-7/pHA-TMZ/TCPP-Lip 可特异性靶向肿瘤细胞，实现高效给药，提高抗肿瘤疗效，降低全身毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.