Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki
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Abstract

Turbulence is one of the least investigated environmental factors impacting the ecophysiology of phytoplankton, both at the community and individual species level. Here, we investigated, for the first time, the effect of a turbulence gradient (Reynolds number, from Reλ = 0 to Reλ = 360) on two species of the marine diatom Pseudo-nitzschia and their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, and Chl a content of P. fraudulenta and P. multiseries were higher for intermediate turbulence (Reλ = 160 or 240). DA was detectable only in P. multiseries samples. These observations were supported by transcriptomic analyses results, which suggested the turbulence related induction of the expression of the DA production locus, with a linkage to an increased photosynthetic activity of the total metatranscriptome. This study also highlighted a higher richness of the bacterial community associated with the nontoxic strain of P. fraudulenta in comparison to the toxic strain of P. multiseries. Bacillus was an important genus in P. multiseries cultures (relative abundance 15.5%) and its highest abundances coincided with the highest DA levels. However, associated bacterial communities of both Pseudo-nitzschia species did not show clear patterns relative to turbulence intensity.

湍流对假尼氏硅藻属硅藻及相关细菌的影响
湍流是影响浮游植物生态生理学的环境因素中最少被研究的因素之一,无论是在群落层面还是个体物种层面。在这里,我们首次研究了湍流梯度(${rm{Reynolds/number}}$,从${\rm{R}}{\rm{e}}}_{rm\{lambda }} = 0$到${rm{R}}{\rm{e}}}_{rm\{lambda }} = 360$)在实验室条件下对两种海洋硅藻假尼茨藻及其相关细菌群落的影响。在中等湍流度(${\rm{R}}{\{rm{e}}}_{\rm{\lambda }} = 160 美元或 240 美元)条件下,欺诈硅藻(P. fraudulenta)和多重硅藻(P. multiseries)的细胞丰度、多莫酸(DA)产量、链形成和 Chl a 含量都较高。只有在P. multiseries样本中才能检测到DA。这些观察结果得到了转录组分析结果的支持,转录组分析结果表明,湍流诱导了 DA 生产基因座的表达,并与总元转录组光合作用活性的增加有关。这项研究还突出表明,与多毒菌属无毒菌株相比,与诈囊藻无毒菌株相关的细菌群落更为丰富。芽孢杆菌(Bacillus)是多毒藻培养物中的重要菌属(相对丰度为 15.5%),其最高丰度与最高 DA 水平相吻合。然而,两种假尼茨藻的相关细菌群落并没有显示出与湍流强度相关的明显模式。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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