Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Cell structure and function Pub Date : 2024-08-27 Epub Date: 2024-08-22 DOI:10.1247/csf.24008
Hirokazu Yagi, Seigo Tateo, Taiki Saito, Yusaku Ohta, Emiko Nishi, Saemi Obitsu, Tatsuya Suzuki, Supaphorn Seetaha, Charles Hellec, Akihiko Nakano, Takuro Tojima, Koichi Kato
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引用次数: 0

Abstract

The Golgi apparatus, a crucial organelle involved in protein processing, including glycosylation, exhibits complex sub-structures, i.e., cis-, medial, and trans-cisternae. This study investigated the distribution of glycosyltransferases within the Golgi apparatus of mammalian cells via 3D super-resolution imaging. Focusing on human glycosyltransferases involved in N-glycan modification, we found that even enzymes presumed to coexist in the same Golgi compartment exhibit nuanced variations in localization. By artificially making their N-terminal regions [composed of a cytoplasmic, transmembrane, and stem segment (CTS)] identical, it was possible to enhance the degree of their colocalization, suggesting the decisive role of this region in determining the sub-Golgi localization of enzymes. Ultimately, this study reveals the molecular codes within CTS regions as key determinants of glycosyltransferase localization, providing insights into precise control over the positioning of glycosyltransferases, and consequently, the interactions between glycosyltransferases and substrate glycoproteins as cargoes in the secretory pathway. This study advances our understanding of Golgi organization and opens avenues for programming the glycosylation of proteins for clinical applications.Key words: Golgi apparatus, glycosyltransferase, 3D super-resolution imaging, N-glycosylation.

通过三维超分辨率成像解密糖基转移酶的亚高尔基定位。
高尔基体是参与蛋白质加工(包括糖基化)的重要细胞器,具有复杂的亚结构,即顺式、中式和反式细胞器。本研究通过三维超分辨率成像研究了哺乳动物细胞高尔基体内糖基转移酶的分布。我们以参与 N-糖修饰的人类糖基转移酶为研究对象,发现即使是被认为共存于同一高尔基区室的酶,其定位也会出现细微的变化。通过人为地使它们的 N 端区域(由胞质、跨膜和茎段(CTS)组成)相同,可以提高它们的共定位程度,这表明该区域在决定酶的高尔基体下定位方面起着决定性作用。最终,这项研究揭示了 CTS 区域内的分子代码是决定糖基转移酶定位的关键因素,为精确控制糖基转移酶的定位,进而控制糖基转移酶与分泌途径中作为货物的底物糖蛋白之间的相互作用提供了见解。这项研究增进了我们对高尔基体组织的了解,并为临床应用中蛋白质的糖基化编程开辟了途径:高尔基体 糖基转移酶 三维超分辨率成像 N-糖基化
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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