{"title":"Application of Caged Sparus aurata in Biomonitoring of the Boka Kotorska Bay (Montenegro)","authors":"Jelena Djordjević Aleksić, Branka Vuković-Gačić, Margareta Kračun-Kolarević, Jovana Jovanović Marić, Zoran Gačić, Rajko Martinović, Danijela Joksimović, Stoimir Kolarević","doi":"10.1007/s11270-024-07316-w","DOIUrl":null,"url":null,"abstract":"<p>In this study early signs of deterioration of the ecosystem in the Boka Kotorska Bay were assessed by applying an active biomonitoring approach for the first time in coastal area of Montenegro. Gilthead sea bream (<i>Sparus aurata</i>), commercially farmed in the Bay, was used as a bioindicator. Specimens were translocated from an aquaculture farm to more impacted sites within the Bay. After two weeks of exposure, the level of DNA damage was assessed in blood cells by comet assay and micronucleus test while muscle tissues were prepared for trace element determination. The results confirmed that an active biomonitoring approach is a valuable tool for eco/geno-toxicological assessment in the marine environment. Comet assay was sensitive in discrimination of the studied groups, moreover it was sensitive enough to detect stress related to the caging procedure and translocation of animals. Lack of feeding during the exposure period was reflected on the condition index. Based on the Integrated Biomarker Response (IBR), the group exposed at the main port - Porto Montenegro was shown to be under higher environmental stress than groups exposed at reference site Dobrota (IBM) and fish farm CogiMAR. An increase in the frequency of micronuclei was evident only at this site (Porto Montenegro). Obtained trace element concentrations in fish tissues were significantly lower in comparison to values recommended by the national legislative.</p>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water, Air, & Soil Pollution","FirstCategoryId":"6","ListUrlMain":"https://doi.org/10.1007/s11270-024-07316-w","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In this study early signs of deterioration of the ecosystem in the Boka Kotorska Bay were assessed by applying an active biomonitoring approach for the first time in coastal area of Montenegro. Gilthead sea bream (Sparus aurata), commercially farmed in the Bay, was used as a bioindicator. Specimens were translocated from an aquaculture farm to more impacted sites within the Bay. After two weeks of exposure, the level of DNA damage was assessed in blood cells by comet assay and micronucleus test while muscle tissues were prepared for trace element determination. The results confirmed that an active biomonitoring approach is a valuable tool for eco/geno-toxicological assessment in the marine environment. Comet assay was sensitive in discrimination of the studied groups, moreover it was sensitive enough to detect stress related to the caging procedure and translocation of animals. Lack of feeding during the exposure period was reflected on the condition index. Based on the Integrated Biomarker Response (IBR), the group exposed at the main port - Porto Montenegro was shown to be under higher environmental stress than groups exposed at reference site Dobrota (IBM) and fish farm CogiMAR. An increase in the frequency of micronuclei was evident only at this site (Porto Montenegro). Obtained trace element concentrations in fish tissues were significantly lower in comparison to values recommended by the national legislative.
期刊介绍:
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.