Serum MicroRNAs as Predictors of Diagnosis and Drug-resistance in Temporal Lobe Epilepsy: A Preliminary Study

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-06-25 DOI:10.2174/1570159x22666240516145823

Gloria Bertoli, Francesco Fortunato, Claudia Cava, Ida Manna, Francesca Gallivanone, Angelo Labate, Antonella Panio, Danilo Porro, Antonio Gambardella

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引用次数: 0

Abstract

Objective: Temporal lobe epilepsy (TLE) is the most common form of refractory focal epilepsy, and the current clinical diagnosis is based on EEG, clinical neurological history and neuroimaging findings. Methods: So far, there are no blood-based molecular biomarkers of TLE to support clinical diagnosis, despite the pathogenic mechanisms underlying TLE involving defects in the regulation of gene expression. MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression. Results: Recent studies show the feasibility of detecting miRNAs in body fluids; circulating miRNAs have emerged as potential clinical biomarkers in epilepsy, although the TLE miRNA profile needs to be addressed. Here, we analysed the diagnostic potential of 8 circulating miRNAs in sera of 52 TLE patients and 40 age- and sex-matched donor controls by RT-qPCR analyses. Conclusion: We found that miR-34a-5p, -106b-5p, -130a-3p, -146a-5p, and -19a-3p are differently expressed in TLE compared to control subjects, suggesting a diagnostic role. Furthermore, we found that miR-34a-5p, -106b-5p, -146a-5p and miR-451a could become prognostic biomarkers, being differentially expressed between drug-resistant and drug-responsive TLE subjects. Therefore, serum miRNAs are diagnostic and drug-resistance predictive molecules of TLE.

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作为颞叶癫痫诊断和耐药性预测因子的血清微RNA：初步研究

目的：颞叶癫痫（TLE）是最常见的难治性局灶性癫痫：颞叶癫痫（TLE）是难治性局灶性癫痫中最常见的一种，目前临床诊断主要依据脑电图、临床神经病史和神经影像学检查结果。方法：尽管 TLE 的致病机制涉及基因表达调控缺陷，但迄今为止还没有基于血液的 TLE 分子生物标志物来支持临床诊断。微RNA（miRNA）已成为基因表达的重要转录后调控因子。结果：最近的研究表明，检测体液中的 miRNAs 是可行的；循环 miRNAs 已成为癫痫的潜在临床生物标志物，但 TLE 的 miRNA 图谱仍有待研究。在这里，我们通过 RT-qPCR 分析了 52 名 TLE 患者和 40 名年龄与性别匹配的供体对照者血清中 8 种循环 miRNA 的诊断潜力。研究结论我们发现，与对照组相比，miR-34a-5p、-106b-5p、-130a-3p、-146a-5p 和 -19a-3p在TLE中的表达量不同，这表明它们在诊断中的作用不同。此外，我们还发现，miR-34a-5p、-106b-5p、-146a-5p 和 miR-451a 可成为预后生物标志物，在耐药和对药物有反应的 TLE 受试者之间有不同的表达。因此，血清miRNA是诊断和预测TLE耐药性的分子。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.