{"title":"Harmonious Operation of PSII Efficiency and Antioxidant Activity is Decisive for Salt Tolerance in Barley Genotypes","authors":"S. Canavar, S. Toksoy Köseoğlu, A. Doğru","doi":"10.1134/s1021443724604737","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this study, the effect of salt stress (120 mM NaCl for 7 d) on some physiological changes was investigated through chlorophyll <i>a</i> fluorescence technique, JIP test, and some endogenous resistance mechanisms (activity of some antioxidant enzymes and free proline content) in two barley cultivars (<i>Hordeum vulgare</i> L. cvs. Erginel-90 and Tokak 157/37). Chlorophyll fluorescence measurements and the calculated JIP test parameters showed that salt stress did not damage PSII reaction centers in both barley cultivars but reduced the rate of electron transport reactions from reaction centers to the plastoquinone pool in the genotype Erginel-90. Moreover, changes in ΔRo and ФRo confirmed that electron transport in the genotype Erginel-90 was also reduced further than plastoquinone. In the genotype Tokak 157/37, however, photosynthetic electron transport reactions were maintained under salt stress conditions. Contrary to the genotype Tokak 157/37, the impairment in the photosynthetic efficiency led to oxidative stress in the leaf tissues of the genotype Erginel-90 under salt stress. This fact was confirmed by the lower foliar antioxidant activity, accumulation of H<sub>2</sub>O<sub>2</sub> and malondialdehyde, and thus photooxidative damages in the photosynthetic pigments. These results allowed us to conclude that the photosynthetic apparatus of the genotype Tokak 157/37 is more tolerant to salt stress than the genotype Erginel-90. In addition, ΔV/Δto, S<sub>M</sub>, N, Ψo, Ψo/(1 – Ψo), PI<sub>ABS</sub>, ФRo, and ΔRo could be considered decisive and informative JIP test parameters to evaluate salt tolerance in barley plants.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"51 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724604737","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
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Abstract
In this study, the effect of salt stress (120 mM NaCl for 7 d) on some physiological changes was investigated through chlorophyll a fluorescence technique, JIP test, and some endogenous resistance mechanisms (activity of some antioxidant enzymes and free proline content) in two barley cultivars (Hordeum vulgare L. cvs. Erginel-90 and Tokak 157/37). Chlorophyll fluorescence measurements and the calculated JIP test parameters showed that salt stress did not damage PSII reaction centers in both barley cultivars but reduced the rate of electron transport reactions from reaction centers to the plastoquinone pool in the genotype Erginel-90. Moreover, changes in ΔRo and ФRo confirmed that electron transport in the genotype Erginel-90 was also reduced further than plastoquinone. In the genotype Tokak 157/37, however, photosynthetic electron transport reactions were maintained under salt stress conditions. Contrary to the genotype Tokak 157/37, the impairment in the photosynthetic efficiency led to oxidative stress in the leaf tissues of the genotype Erginel-90 under salt stress. This fact was confirmed by the lower foliar antioxidant activity, accumulation of H2O2 and malondialdehyde, and thus photooxidative damages in the photosynthetic pigments. These results allowed us to conclude that the photosynthetic apparatus of the genotype Tokak 157/37 is more tolerant to salt stress than the genotype Erginel-90. In addition, ΔV/Δto, SM, N, Ψo, Ψo/(1 – Ψo), PIABS, ФRo, and ΔRo could be considered decisive and informative JIP test parameters to evaluate salt tolerance in barley plants.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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