J. J. Hu, H. Song, Z. H. Cao, Q. L. Shu, B. X. Han
{"title":"Comparative Analysis of Transcriptome at Different Growth and Development Stages of Camellia oleifera","authors":"J. J. Hu, H. Song, Z. H. Cao, Q. L. Shu, B. X. Han","doi":"10.1134/s1021443724604488","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>To investigate the gene expression and metabolic pathways involved in flower development at different growth stages of <i>Camellia oleifera</i>, this study focused on leaves collected during the juvenile stage, initial fruiting stage, and peak fruiting stage of the ‘Dabieshan 1’ <i>C. oleifera</i> variety. Transcriptome sequencing analysis was performed to obtain the transcriptome data of leaf samples during the pre-flowering period. The annotated genes included key regulators of flower development pathways, such as <i>FLOWERING LOCUS C</i> (<i>FLC)</i>, <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1</i> (<i>SOC1)</i>, <i>SUPPRESSOR OVEREXPRESSION CONSTANS 2</i> (<i>SOC2)</i>, as well as genes involved in the photoperiod pathway, including <i>CYCLING DOF FACTOR 2</i> (<i>CDF2)</i>, <i>CYCLING DOF FACTOR 3</i> (<i>CDF3)</i>, and <i>LATE ELONGATED HYPOCOTYL</i> (<i>LHY)</i>. Specifically, 7 UniGene sequences of the <i>CDF2</i> gene, 3 UniGene sequences of the <i>CDF3</i> gene, and 3 UniGene sequences of the <i>LHY</i> gene were identified, highlighting their important roles in flower development regulation in <i>C. oleifera</i>. Gene Ontology analysis demonstrated enrichment in various biological processes related to carbohydrate metabolism, biosynthesis, and environmental response, while differentially expressed genes in the molecular function category were associated with degradation activities. In terms of cellular components, the differentially expressed genes were enriched in ribosome and plasma membranes. Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment in multiple metabolic pathways, biosynthesis, and degradation across the three developmental stages of <i>C. oleifera</i> leaves. These findings provide a foundation for further investigation into the energy metabolism involved in the regulation of flower development in <i>C. oleifera</i>.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"367 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724604488","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
To investigate the gene expression and metabolic pathways involved in flower development at different growth stages of Camellia oleifera, this study focused on leaves collected during the juvenile stage, initial fruiting stage, and peak fruiting stage of the ‘Dabieshan 1’ C. oleifera variety. Transcriptome sequencing analysis was performed to obtain the transcriptome data of leaf samples during the pre-flowering period. The annotated genes included key regulators of flower development pathways, such as FLOWERING LOCUS C (FLC), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), SUPPRESSOR OVEREXPRESSION CONSTANS 2 (SOC2), as well as genes involved in the photoperiod pathway, including CYCLING DOF FACTOR 2 (CDF2), CYCLING DOF FACTOR 3 (CDF3), and LATE ELONGATED HYPOCOTYL (LHY). Specifically, 7 UniGene sequences of the CDF2 gene, 3 UniGene sequences of the CDF3 gene, and 3 UniGene sequences of the LHY gene were identified, highlighting their important roles in flower development regulation in C. oleifera. Gene Ontology analysis demonstrated enrichment in various biological processes related to carbohydrate metabolism, biosynthesis, and environmental response, while differentially expressed genes in the molecular function category were associated with degradation activities. In terms of cellular components, the differentially expressed genes were enriched in ribosome and plasma membranes. Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment in multiple metabolic pathways, biosynthesis, and degradation across the three developmental stages of C. oleifera leaves. These findings provide a foundation for further investigation into the energy metabolism involved in the regulation of flower development in C. oleifera.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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