Koung Hee Kim, Mi-Ran Ki, Thi Khoa My Nguyen, Ki Ha Min, Seung Pil Pack
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引用次数: 0
Abstract
Ferritin (Fn) is an attractive nano-biomaterial with a hollow structure. Functional chimeric cage-protein Fns (chimera-Fn) can be designed by combining two different monomer subunits. However, unavoidable damage is induced by the severe disassembly conditions required for preparing the Fn monomer. Here, a biocompatible method using a designed dual-expression vector to obtain chimera-Fns without a damage-inducing process is described. Fn monomer and silica-forming peptide (Kpt)-fused Fn monomer are expressed simultaneously and assembled within host-cells to form chimera-Fn of Kpt. Characteristic analysis showed that the chimera-Fns obtained intracellularly were composed of the two monomers in a ratio of 1:1. Morphological and functional analyses determined that the intracellularly-generated chimera-Fns had more intact forms and bioactive functions compared with those produced by chemical-involved disassembly and reassembly. This is the first report about the self-assembly of chimera-Fn with intact function and hold promise in designing functional templates for various nanomaterial preparations.
期刊介绍:
Journal of Industrial and Engineering Chemistry is published monthly in English by the Korean Society of Industrial and Engineering Chemistry. JIEC brings together multidisciplinary interests in one journal and is to disseminate information on all aspects of research and development in industrial and engineering chemistry. Contributions in the form of research articles, short communications, notes and reviews are considered for publication. The editors welcome original contributions that have not been and are not to be published elsewhere. Instruction to authors and a manuscript submissions form are printed at the end of each issue. Bulk reprints of individual articles can be ordered. This publication is partially supported by Korea Research Foundation and the Korean Federation of Science and Technology Societies.