Ultrabroadband two-beam coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy of organic fluids: A comparative study

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Timea Koch, Roland Ackermann, Axel Stoecker, Tobias Meyer-Zedler, Thomas Gabler, Tom Lippoldt, Jeannine Missbach-Guentner, Christoph Russmann, Jürgen Popp, Stefan Nolte
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Abstract

Spontaneous Raman spectroscopy is a well-established diagnostic tool, allowing for the identification of all Raman active species with a single measurement. Yet, it may suffer from low-signal intensity and fluorescent background. In contrast, coherent anti-Stokes Raman scattering (CARS) offers laser-like signals, but the traditional approach lacks the multiplex capability of spontaneous Raman spectroscopy. We present an ultrabroadband CARS setup which aims at exciting the full spectrum (300–3700 cm−1) of biological molecules. A dual-output optical parametric amplifier provides a ~7 fs pump/Stokes and a ~700 fs probe pulse. CARS spectra of DMSO, ethanol, and methanol show great agreement with spontaneous Raman spectroscopy and superiority in fluorescent environments. The spectral resolution proves sufficient to differentiate between the complex spectra of L-proline and hydroxyproline. Moreover, decay constants in the sub picosecond range are determined for individual Raman transitions, providing an additional approach for sample characterization.

Abstract Image

有机流体的超宽带双光束相干反斯托克斯拉曼散射和自发拉曼光谱:比较研究。
自发拉曼光谱是一种成熟的诊断工具,只需一次测量就能识别所有拉曼活性物种。然而,它可能存在信号强度低和荧光背景的问题。相比之下,相干反斯托克斯拉曼散射(CARS)可提供类似激光的信号,但这种传统方法缺乏自发拉曼光谱的复用能力。我们提出了一种超宽带 CARS 装置,旨在激发生物分子的全光谱(300-3700 cm-1)。双输出光参量放大器提供约 7 fs 的泵浦/斯托克斯脉冲和约 700 fs 的探测脉冲。二甲基亚砜、乙醇和甲醇的 CARS 光谱与自发拉曼光谱非常吻合,在荧光环境中也非常优越。光谱分辨率足以区分 L-脯氨酸和羟脯氨酸的复杂光谱。此外,还确定了单个拉曼跃迁在亚皮秒范围内的衰减常数,为样品表征提供了另一种方法。
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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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