The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia.

IF 5.1 Q2 CELL BIOLOGY
Simonetta Falzoni, Valentina Vultaggio-Poma, Paola Chiozzi, Mario Tarantini, Elena Adinolfi, Paola Boldrini, Anna Lisa Giuliani, Giampaolo Morciano, Yong Tang, Dariusz C Gorecki, Francesco Di Virgilio
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引用次数: 0

Abstract

Microparticles (MPs) are secreted by all cells, where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2X7 receptor (P2X7R) activation by extracellular ATP (eATP) causes a large MP release and affects their contents in a cell-specific fashion. We investigated MP release and functional impact in microglial cells from P2X7R-WT or P2X7R-KO mice, as well as mouse microglial cell lines characterized for high (N13-P2X7RHigh) or low (N13-P2X7RLow) P2X7R expression. P2X7R stimulation promoted release of a mixed MP population enriched with naked mitochondria. Released mitochondria were taken up and incorporated into the mitochondrial network of the recipient cells in a P2X7R-dependent fashion. NLRP3 and the P2X7R itself were also delivered to the recipient cells. Microparticle transfer increased the energy level of the recipient cells and conferred a pro-inflammatory phenotype. These data show that the P2X7R is a master regulator of intercellular organelle and MP trafficking in immune cells.

P2×7 受体是小鼠小胶质细胞微粒和线粒体交换的主调节器。
微颗粒(MPs)由所有细胞分泌,在细胞间通信、分化、炎症和细胞能量转移中发挥着关键作用。细胞外 ATP(eATP)激活 P2×7 受体(P2×7R)会导致大量 MP 释放,并以细胞特异的方式影响其内容。我们研究了来自 P2×7R-WT 或 P2×7R-KO 小鼠以及高(N13-P2×7RHigh)或低(N13-P2×7RLow)P2×7R 表达的小鼠小胶质细胞系的 MP 释放和功能影响。P2×7R 刺激促进了富含裸线粒体的混合 MP 群体的释放。释放的线粒体以 P2×7R 依赖性方式被吸收并整合到受体细胞的线粒体网络中。NLRP3 和 P2×7R 本身也被输送到受体细胞。MP 转移提高了受体细胞的能量水平,并产生了促炎表型。这些数据表明,P2×7R 是免疫细胞中细胞器间和 MP 转运的主调控因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.70
自引率
0.00%
发文量
0
审稿时长
3 weeks
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