Differential phagocytic expression of IC-21 macrophages and their scavenging receptors during inflammatory induction by oxysterol: A microscopic approach

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-07-10 DOI:10.1002/jemt.24647

Parimalanandhini Duraisamy, Sangeetha Ravi, Livya Catherene Martin, Manikandan Kumaresan, Beulaja Manikandan, Manikandan Ramar

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Abstract

Phagocytosis by macrophages dates back to a long history in science, this present study deals with new approaches that have been analyzed and standardized towards the interesting aspects of primary and secondary macrophages. The distinct morphological differences in primary and secondary phagocytic cells were observed and the phagocytic response of secondary macrophages under the influence of 7-ketocholesterol and lipopolysaccharide was analyzed. The primary peritoneal and secondary IC-21 cells unveiled explicit differences in nuclear numbers shapes and sizes of the granules present within the cytoplasmic region. Further, potent inducers 7KCh and LPS influenced an effective activation of IC-21 macrophages and resulted in ROS generation, irregulated protein expressions of CD86, CD68, and CD206 with enhanced phagocytic responses towards goat, cow, and human RBC targets with significant phagocytic rate and index were observed. Moreover, a remarkable observation of target specificity and aggregations with IC-21 phagocytic macrophages revealed the notion that specific membrane receptors and secretory molecules (lysosomes) are primarily involved in their phagocytic mechanism.

Research Highlights

IC-21 macrophages are peritoneal origin from mice but the primary peritoneal macrophages and cell line show distinct differences.
IC-21 macrophages express target-specific phagocytosis.
Phagocytosis in IC-21 macrophages is regulated by CD markers (68, 86, and 206).

Abstract Image

查看原文本刊更多论文

氧杂环醇诱发炎症过程中 IC-21 巨噬细胞及其清除受体的不同吞噬表达：显微镜方法。

巨噬细胞的吞噬作用在科学界有着悠久的历史，本研究采用新方法对初级和次级巨噬细胞的有趣方面进行了分析和标准化。本研究观察了原发性和继发性吞噬细胞的明显形态差异，并分析了继发性巨噬细胞在 7-酮胆固醇和脂多糖影响下的吞噬反应。原发性腹膜细胞和继发性 IC-21 细胞在核数量、细胞质区内颗粒的形状和大小方面存在明显差异。此外，强效诱导剂 7KCh 和 LPS 影响了 IC-21 巨噬细胞的有效活化，并导致 ROS 生成，CD86、CD68 和 CD206 蛋白表达紊乱，对山羊、牛和人红细胞靶标的吞噬反应增强，吞噬率和吞噬指数显著提高。此外，IC-21吞噬巨噬细胞的目标特异性和聚集性的显著观察结果表明，特定的膜受体和分泌分子（溶酶体）主要参与了它们的吞噬机制。研究亮点IC-21 巨噬细胞来源于小鼠腹膜，但腹膜原代巨噬细胞和细胞系表现出明显的差异。IC-21 巨噬细胞具有目标特异性吞噬功能。IC-21 巨噬细胞的吞噬作用受 CD 标记（68、86 和 206）的调控。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.