Unveiling the limits of precision in iterative MINFLUX.

IF 1.5 4区工程技术 Q3 MICROSCOPY

Journal of microscopy Pub Date : 2024-07-10 DOI:10.1111/jmi.13338

Carlas Smith, Dylan Kalisvaart, Kirti Prakash

引用次数: 0

Abstract

In single-molecule microscopy, a big question is how precisely we can estimate the location of a single molecule. Our research shows that by using iterative localisation microscopy and factoring in the prior information, we can boost precision and reduce the number of photons needed. Leveraging the Van Trees inequality aids in determining the optimal precision achievable. Our approach holds promise for wider application in discerning the optimal precision across diverse imaging scenarios, encompassing various illumination strategies, point spread functions and overarching control methodologies.

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揭示迭代 MINFLUX 的精度极限。

在单分子显微镜中，一个很大的问题是我们如何才能精确地估计出单分子的位置。我们的研究表明，通过使用迭代定位显微镜并将先验信息考虑在内，我们可以提高精度并减少所需的光子数量。范特里不等式有助于确定可达到的最佳精度。我们的方法有望更广泛地应用于各种成像方案，包括各种照明策略、点扩散函数和总体控制方法，以确定最佳精度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microscopy 工程技术-显微镜技术

CiteScore

4.30

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.