Unveiling the limits of precision in iterative MINFLUX

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-10 DOI:10.1111/jmi.13338

Carlas Smith, Dylan Kalisvaart, Kirti Prakash

引用次数: 0

Abstract

In single-molecule microscopy, a big question is how precisely we can estimate the location of a single molecule. Our research shows that by using iterative localisation microscopy and factoring in the prior information, we can boost precision and reduce the number of photons needed. Leveraging the Van Trees inequality aids in determining the optimal precision achievable. Our approach holds promise for wider application in discerning the optimal precision across diverse imaging scenarios, encompassing various illumination strategies, point spread functions and overarching control methodologies.

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揭示迭代 MINFLUX 的精度极限。

在单分子显微镜中，一个很大的问题是我们如何才能精确地估计出单分子的位置。我们的研究表明，通过使用迭代定位显微镜并将先验信息考虑在内，我们可以提高精度并减少所需的光子数量。范特里不等式有助于确定可达到的最佳精度。我们的方法有望更广泛地应用于各种成像方案，包括各种照明策略、点扩散函数和总体控制方法，以确定最佳精度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.