Tear fluid cytokine analysis: a non-invasive approach for assessing retinopathy of prematurity severity.

IF 2.1 3区医学 Q2 OPHTHALMOLOGY

Japanese Journal of Ophthalmology Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI:10.1007/s10384-024-01084-0

Takashi Baba, Ryu Uotani, Kodai Inata, Shin-Ichi Sasaki, Yumiko Shimizu, Mazumi Miura, Yoshitsugu Inoue, Dai Miyazaki

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引用次数: 0

Abstract

Purpose: To determine whether there is a significant association between inflammatory cytokines in the tear fluid and the severity of Retinopathy of Prematurity (ROP).

Study design: Retrospective cohort study.

Methods: The cytokine levels in tear fluids were determined in 34 eyes with ROP and 18 eyes without ROP. There were 15 eyes with severe ROP requiring treatment and 19 eyes with mild ROP not requiring treatment. For severe ROP eyes, tear fluids were collected before treatment.

Results: Significantly higher levels of CCL2 and vascular endothelial growth factor (VEGF) were detected in eyes with severe ROP compared to eyes with mild ROP and no ROP. When assessed for cytokine levels that discriminate each disease group, CCL2 showed a significant odds ratio of 1.76 for severity change (/quintile, P = 0.032, after adjusting for birth weight). Correlation analysis showed that birth weight correlated with IL-1α levels, and decreased weight gain increased IFN-γ levels. We next determined tear fluid cytokines which discriminate severe ROP using receiver operating characteristics' analysis. We found that combination of higher CCL2 levels, higher VEGF levels, and lower IFN-γ levels in the tear fluid had a stronger predictive value for severe ROP (area under curve, 0.85).

Conclusion: The levels of CCL2, VEGF, and IFN-γ in tear fluid may serve as useful biomarkers for assessing the severity of ROP.

Abstract Image

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泪液细胞因子分析：评估早产儿视网膜病变严重程度的无创方法。

目的：确定泪液中的炎性细胞因子与早产儿视网膜病变（ROP）的严重程度是否存在显著关联：研究设计：回顾性队列研究：方法：对 34 只患有早产儿视网膜病变的眼睛和 18 只未患有早产儿视网膜病变的眼睛的泪液中的细胞因子水平进行测定。其中 15 只眼睛患有需要治疗的重度 ROP，19 只眼睛患有不需要治疗的轻度 ROP。重度 ROP 患者的泪液在治疗前采集：结果：与轻度 ROP 和未患 ROP 的眼睛相比，重度 ROP 眼睛的 CCL2 和血管内皮生长因子 (VEGF) 水平明显更高。在评估可区分各疾病组的细胞因子水平时，CCL2 与严重程度变化的几率比为 1.76（/五分位数，P = 0.032，调整出生体重后）。相关分析表明，出生体重与 IL-1α 水平相关，而体重增加会增加 IFN-γ 水平。接下来，我们利用接收器操作特征分析确定了可区分严重 ROP 的泪液细胞因子。我们发现，泪液中 CCL2 水平较高、VEGF 水平较高和 IFN-γ 水平较低的组合对重度 ROP 有更强的预测价值（曲线下面积为 0.85）：结论：泪液中的 CCL2、血管内皮生长因子和 IFN-γ 水平可作为评估 ROP 严重程度的有用生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Japanese Journal of Ophthalmology 医学-眼科学

CiteScore

4.80

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Japanese Journal of Ophthalmology (JJO) was inaugurated in 1957 as a quarterly journal published in English by the Ophthalmology Department of the University of Tokyo, with the aim of disseminating the achievements of Japanese ophthalmologists worldwide. JJO remains the only Japanese ophthalmology journal published in English. In 1997, the Japanese Ophthalmological Society assumed the responsibility for publishing the Japanese Journal of Ophthalmology as its official English-language publication. Currently the journal is published bimonthly and accepts papers from authors worldwide. JJO has become an international interdisciplinary forum for the publication of basic science and clinical research papers.