Mancozeb induces cytogenotoxicity in meristematic cells of Allium cepa L.

IF 2.1 4区 医学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Drug and Chemical Toxicology Pub Date : 2025-05-01 Epub Date: 2024-07-10 DOI:10.1080/01480545.2024.2370938
Gabriel Osvair Costa Jardim, George Laylson da Silva Oliveira
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引用次数: 0

Abstract

Mancozeb is a fungicide of the dithiocarbamate functional group, and it is widely used in agriculture to control various fungal diseases. Thus, studies detailing its toxicological characteristics are necessary, as the population may be exposed through the consumption of food or water contaminated with mancozeb. The aim of this study was to evaluate the cytotoxic, genotoxic, and mutagenic potentials of this dithiocarbamate using the Allium cepa L. test system as well as its cytotoxicity in erythrocytes of female rats (Rattus norvegicus). The meristematic roots of A. cepa bulbs were exposed to various concentrations of mancozeb (62.5, 125, 250, and 500 mg/L) for 24, 48, and 72 h to determine cytotoxicity by evaluating the mitotic index (MI), chromosomal aberrations (CA), and nuclear anomalies (NA) for genotoxicity analysis and micronuclei (MN) for mutagenicity analysis. Distilled water and copper sulfate (0.0006 mg/L) were used as the negative control (NC) and positive control (PC), respectively. The MI and the sum of CA and NA of all the mancozeb concentrations showed a significant difference (p ≤ 0.05) in relation to the NC, indicating possible cytotoxicity and genotoxicity induced by mancozeb. Additionally, MN significantly increased with mancozeb concentration from 250 mg/L to 500 mg/L in 24 h when compared to NC. In another study model, mancozeb showed to be cytolytic at concentrations starting from 125 mg/L. Therefore, these results indicate that mancozeb causes cytogenetic alterations and mutagenicity at lower concentrations than those used in agriculture, which emphasizes the need for more care when managing this fungicide.

代森锰锌诱导薤白分生细胞的细胞遗传毒性
代森锰锌是一种二硫代氨基甲酸酯类杀菌剂,在农业中广泛用于控制各种真菌疾病。因此,有必要对其毒理学特性进行详细研究,因为人们可能会通过食用被代森锰锌污染的食物或水而接触到它。本研究的目的是利用 Allium cepa L. 试验系统评估这种二硫代氨基甲酸酯的细胞毒性、遗传毒性和诱变潜力,以及它对雌性大鼠(Rattus norvegicus)红细胞的细胞毒性。将芒克唑暴露于不同浓度(62.5、125、250 和 500 毫克/升)的鳞茎分生根中 24、48 和 72 小时,通过评估有丝分裂指数(MI)、染色体畸变(CA)和核异常(NA)(用于遗传毒性分析)和微核(MN)(用于诱变性分析)来确定细胞毒性。蒸馏水和硫酸铜(0.0006 毫克/升)分别用作阴性对照(NC)和阳性对照(PC)。与 NC 相比,所有浓度的芒克唑的 MI 以及 CA 和 NA 之和均有显著差异(p ≤ 0.05),这表明芒克唑可能具有细胞毒性和遗传毒性。此外,与 NC 相比,在 24 小时内,随着芒克泽浓度从 250 mg/L 升至 500 mg/L,MN 明显增加。在另一个研究模型中,锰锌浓度从 125 毫克/升开始就显示出细胞溶解作用。因此,这些结果表明,与农业中使用的浓度相比,锰锌在较低浓度下就会导致细胞遗传改变和诱变性,这强调了在管理这种杀真菌剂时需要更加小心。
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来源期刊
Drug and Chemical Toxicology
Drug and Chemical Toxicology 医学-毒理学
CiteScore
6.00
自引率
3.80%
发文量
99
审稿时长
3 months
期刊介绍: Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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