Dermatoprotective effect of Moringa oleifera leaf extract on sodium valproate-induced skin damage in rats.

IF 2.1 4区 医学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Drug and Chemical Toxicology Pub Date : 2024-11-01 Epub Date: 2024-07-10 DOI:10.1080/01480545.2024.2369586
Gülsüm Elik, Sehkar Oktay, Ismet Burcu Turkyilmaz, Burcin Alev-Tuzuner, Umar Faruk Magaji, Ozlem Sacan, Refiye Yanardag, Aysen Yarat
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Abstract

Valproic acid is an antiepileptic drug associated with skin-related issues like excessive hair growth, hair loss, and skin rashes. In contrast, Moringa oleifera, rich in nutrients and antioxidants, is gaining popularity worldwide for its medicinal properties. The protective properties of M. oleifera extract against skin-related side effects caused by valproic acid were investigated. Female rats were divided into control groups and experimental groups such as moringa, sodium valproate, and sodium valproate + moringa groups. A 70% ethanolic extract of moringa (0.3 g/kg/day) was given to moringa groups, and a single dose of sodium valproate (0.5 g/kg/day) was given to valproate groups for 15 days. In the skin samples, antioxidant parameters (such as glutathione, glutathione-S-transferase, superoxide dismutase, catalase, and total antioxidant capacity), as well as oxidant parameters representing oxidative stress (i.e. lipid peroxidation, sialic acid, nitric oxide, reactive oxygen species, and total oxidant capacity), were examined. Additionally, boron, hydroxyproline, sodium-potassium ATPase, and tissue factor values were determined. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was also carried out for protein analysis in the skin samples. The results showed that moringa could increase glutathione, total antioxidant capacity, sodium-potassium ATPase, and boron levels, while decreasing lipid peroxidation, sialic acid, nitric oxide, total oxidant capacity, reactive oxygen species, hydroxyproline, and tissue factor levels. These findings imply that moringa possesses the potential to mitigate dermatological side effects.

辣木叶提取物对丙戊酸钠引起的大鼠皮肤损伤的皮肤保护作用
丙戊酸是一种抗癫痫药物,与毛发过度生长、脱发和皮疹等皮肤相关问题有关。与此相反,富含营养和抗氧化剂的油辣木因其药用特性而受到全世界的欢迎。本实验研究了油橄榄提取物对丙戊酸引起的皮肤副作用的保护作用。雌性大鼠被分为对照组和实验组,如吗啉草组、丙戊酸钠组和丙戊酸钠 + 吗啉草组。对照组大鼠服用 70% 的吗啉草乙醇提取物(0.3 克/千克/天),丙戊酸钠组大鼠服用单剂量丙戊酸钠(0.5 克/千克/天),连续服用 15 天。对皮肤样本中的抗氧化剂参数(如谷胱甘肽、谷胱甘肽-S-转移酶、超氧化物歧化酶、过氧化氢酶和总抗氧化能力)以及代表氧化应激的氧化剂参数(即脂质过氧化、硅氨酸、一氧化氮、活性氧和总氧化能力)进行了检测。此外,还测定了硼、羟脯氨酸、钠钾 ATP 酶和组织因子值。还对皮肤样本中的蛋白质进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析。结果表明,辣木可以提高谷胱甘肽、总抗氧化能力、钠钾 ATP 酶和硼的水平,同时降低脂质过氧化、硅酸、一氧化氮、总氧化能力、活性氧、羟脯氨酸和组织因子的水平。这些研究结果表明,辣木具有减轻皮肤病副作用的潜力。
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来源期刊
Drug and Chemical Toxicology
Drug and Chemical Toxicology 医学-毒理学
CiteScore
6.00
自引率
3.80%
发文量
99
审稿时长
3 months
期刊介绍: Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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