Effect of RNAi mediated silencing of DIB, JHE, and CAM on the diapause termination of Calliptamus italicus (Orthoptera: Acrididae) eggs.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-07-10 DOI:10.1002/ps.8308

Na Zhao, Wei He, Hongxia Hu, Xuefeng Lv, Fei Yu, Rong Ji, Xiaofang Ye

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Abstract

Background: Calliptamus italicus L. is a major pest in Xinjiang grassland. The diapause overwintering strategy is one of the important reasons for the large population of this pest. This study investigated the function of the genes associated with the release of diapause (DIB, JHE and CAM) in Calliptamus italicus by RNA interference (RNAi) technology to aid in its biological control.

Results: The expression levels of DIB and its downstream-associated genes (EcR and FTZ-F1) in the eggs injected with dsDIB for 12 h decreased by 96.6%, 55.8% and 81.8%, respectively. Diapause began to terminate on day 3, and development was almost complete on day 6. However, the head was significantly smaller. The expression levels of JHE and its downstream-associated genes (JHEH and VgR) at 48 h after dsJHE treatment decreased by 76.5%, 85.6% and 85.9%, respectively. The termination of diapause occured on day 3 of incubation. The development was basically complete on day 6, but the yolk had been incompletely absorbed. The expression of CAM and its downstream-associated genes (CAMK4 and MYL) at 24 h after dsCAM treatment decreased by 42.4%, 95.3% and 82.7%, respectively. Diapause termination was completed on day 4 for incubation, and development was abnormal on day 6. The absorption of yolk was incomplete.

Conclusion: DIB, JHE and CAM can delay the diapause termination of Calliptamus italicus eggs to different degrees and can be developed as potential target genes for its biological control. © 2024 Society of Chemical Industry.

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RNAi 介导的 DIB、JHE 和 CAM 沉默对 Italicus Calliptamus (Orthoptera: Acrididae) 卵休眠终止的影响。

背景褐飞虱是新疆草原上的一种主要害虫。冬眠越冬是该害虫大量繁殖的重要原因之一。本研究通过RNA干扰（RNAi）技术研究了Calliptamus italicus体内与休眠释放相关的基因（DIB、JHE和CAM）的功能，以帮助对其进行生物防治：结果：注射dsDIB 12小时的卵中，DIB及其下游相关基因（EcR和FTZ-F1）的表达水平分别下降了96.6%、55.8%和81.8%。停滞期在第 3 天开始终止，发育在第 6 天基本完成。但头部明显变小。dsJHE处理后48小时，JHE及其下游相关基因（JHEH和VgR）的表达水平分别下降了76.5%、85.6%和85.9%。休眠期在培养的第 3 天结束。第 6 天发育基本完成，但卵黄尚未完全吸收。dsCAM处理后24小时，CAM及其下游相关基因（CAMK4和MYL）的表达量分别下降了42.4%、95.3%和82.7%。孵化第 4 天停滞终止，第 6 天发育异常。结论：结论：DIB、JHE 和 CAM 能在不同程度上延迟草履虫卵的休眠终止，可作为潜在的目标基因进行生物防治。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.

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