{"title":"High-spatiotemporal resolution microwave-induced thermoacoustic tomography for imaging biological dynamics in deep tissue","authors":"Yu Wang, Xiaoyu Tang, Huan Qin","doi":"10.1063/5.0216061","DOIUrl":null,"url":null,"abstract":"Biological systems undergo constant dynamic changes across various spatial and temporal scales. To investigate the intricate biological dynamics in living organisms, there is a strong need for high-speed and high-resolution imaging capabilities with significant imaging depth. In this work, we present high-spatiotemporal resolution microwave-induced thermoacoustic tomography (HR-MTAT) as a method for imaging biological dynamics in deep tissues. HR-MTAT utilizes nanosecond pulsed microwave excitation and ultrasound detection, with appropriate spatial configurations, to achieve high coupling of the sample to the microwaves, to produce images in soft tissue with dielectric contrast and sub-millimeter spatial resolution (230 μm), to a depth of a few centimeters. Notably, by employing a 128-channel parallel signal acquisition and digitization strategy, the field programmable gate array module manages data synthesis, and GPU-based parallel pixel reconstruction facilitates HR-MTAT to accomplish single-frame image reconstruction in an impressive 50 μs. The practical feasibility of HR-MTAT was evaluated in live mice. The results show that HR-MTAT can noninvasively image whole-body small animals (up to 60 mm in depth) with clear resolution of internal organ structures at a frame rate of 100 Hz, without the need for labeling. At this high spatiotemporal resolution, HR-MTAT can capture respiration, heartbeat, and arterial pulse propagation without motion artifacts and track bio-nanoprobes in livers and tumors. These findings demonstrate HR-MTAT's ability to perform dynamic imaging with high contrast and resolution in deep tissues.","PeriodicalId":8094,"journal":{"name":"Applied Physics Letters","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Physics Letters","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1063/5.0216061","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHYSICS, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Biological systems undergo constant dynamic changes across various spatial and temporal scales. To investigate the intricate biological dynamics in living organisms, there is a strong need for high-speed and high-resolution imaging capabilities with significant imaging depth. In this work, we present high-spatiotemporal resolution microwave-induced thermoacoustic tomography (HR-MTAT) as a method for imaging biological dynamics in deep tissues. HR-MTAT utilizes nanosecond pulsed microwave excitation and ultrasound detection, with appropriate spatial configurations, to achieve high coupling of the sample to the microwaves, to produce images in soft tissue with dielectric contrast and sub-millimeter spatial resolution (230 μm), to a depth of a few centimeters. Notably, by employing a 128-channel parallel signal acquisition and digitization strategy, the field programmable gate array module manages data synthesis, and GPU-based parallel pixel reconstruction facilitates HR-MTAT to accomplish single-frame image reconstruction in an impressive 50 μs. The practical feasibility of HR-MTAT was evaluated in live mice. The results show that HR-MTAT can noninvasively image whole-body small animals (up to 60 mm in depth) with clear resolution of internal organ structures at a frame rate of 100 Hz, without the need for labeling. At this high spatiotemporal resolution, HR-MTAT can capture respiration, heartbeat, and arterial pulse propagation without motion artifacts and track bio-nanoprobes in livers and tumors. These findings demonstrate HR-MTAT's ability to perform dynamic imaging with high contrast and resolution in deep tissues.
期刊介绍:
Applied Physics Letters (APL) features concise, up-to-date reports on significant new findings in applied physics. Emphasizing rapid dissemination of key data and new physical insights, APL offers prompt publication of new experimental and theoretical papers reporting applications of physics phenomena to all branches of science, engineering, and modern technology.
In addition to regular articles, the journal also publishes invited Fast Track, Perspectives, and in-depth Editorials which report on cutting-edge areas in applied physics.
APL Perspectives are forward-looking invited letters which highlight recent developments or discoveries. Emphasis is placed on very recent developments, potentially disruptive technologies, open questions and possible solutions. They also include a mini-roadmap detailing where the community should direct efforts in order for the phenomena to be viable for application and the challenges associated with meeting that performance threshold. Perspectives are characterized by personal viewpoints and opinions of recognized experts in the field.
Fast Track articles are invited original research articles that report results that are particularly novel and important or provide a significant advancement in an emerging field. Because of the urgency and scientific importance of the work, the peer review process is accelerated. If, during the review process, it becomes apparent that the paper does not meet the Fast Track criterion, it is returned to a normal track.