Influence of acute heat shock on antioxidant defense of tropical fish, Psalidodon bifasciatus

IF 1.9 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ana Paula Nascimento Corrêa , Niumaique Gonçalves da Silva , Jonathan Ratko , Diego Ortiz da Silva , Ieda Cristina Schleger , Diego Mauro Carneiro Pereira , Ananda Karla Alves Neundorf , Maria Rosa Dmengeon Pedreiro de Souza , Tatiana Herrerias , Lucélia Donatti
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Abstract

Psalidodon bifasciatus is a fish species sensitive to physical and chemical changes in water. It serves as a good bioindicator of temperature variations and is utilized in environmental monitoring studies in Brazilian rivers. The objective of this study was to evaluate antioxidant defense biomarkers in the heart, brain, and muscle of P. bifasciatus exposed to a 10 °C thermal increase. P. bifasciatus were collected and divided into a control group (21 °C) and groups subjected to thermal shock (31 °C) for periods of 2, 6, 12, 24, and 48h. Two-way ANOVA indicated that a 10 °C temperature increase caused oxidative stress in P. bifasciatus. This was evidenced by altered levels of lipid peroxidation (LPO), carbonylated proteins (PCO), and glutathione peroxidase (GPx) in the heart, catalase (CAT) and LPO in the brain, and LPO in the muscle. Principal component analysis (PCA) and integrated biomarker response (IBR) analysis indicated that, compared to the heart and muscle, the brain exhibited a greater activation of the antioxidant response. Sensitivity analysis indicated that the muscle was the most sensitive organ, followed by the brain and heart. Our results indicate that the stress response is tissue-specific through the activation of distinct mechanisms. These responses may be associated with the tissue's function as well as its energy demand. As expected, P. bifasciatus showed changes in response to thermal stress, with the brain showing the greatest alteration in antioxidant defenses and the muscle being the most sensitive tissue.

Abstract Image

急性热休克对热带鱼抗氧化防御的影响
Psalidodon bifasciatus 是一种对水中物理和化学变化很敏感的鱼类。它是温度变化的良好生物指标,被用于巴西河流的环境监测研究。本研究的目的是评估暴露于 10 °C热升温环境中的双吻鲈(P. bifasciatus)心脏、大脑和肌肉中的抗氧化防御生物标志物。收集双尾鲈并将其分为对照组(21 °C)和受热冲击组(31 °C),受热冲击时间分别为 2、6、12、24 和 48 小时。双向方差分析结果表明,温度升高 10 °C会导致双尾蝇产生氧化应激。具体表现为心脏中脂质过氧化物(LPO)、羰基化蛋白质(PCO)和谷胱甘肽过氧化物酶(GPx)水平的变化,大脑中过氧化氢酶(CAT)和 LPO 水平的变化,以及肌肉中 LPO 水平的变化。主成分分析(PCA)和综合生物标记反应(IBR)分析表明,与心脏和肌肉相比,大脑的抗氧化反应激活程度更高。敏感性分析表明,肌肉是最敏感的器官,其次是大脑和心脏。我们的研究结果表明,应激反应通过激活不同的机制而具有组织特异性。这些反应可能与组织的功能及其能量需求有关。正如预期的那样,双尾蛙对热应力的反应发生了变化,大脑的抗氧化防御能力变化最大,肌肉则是最敏感的组织。
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来源期刊
CiteScore
4.60
自引率
4.50%
发文量
77
审稿时长
22 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part B: Biochemical and Molecular Biology (CBPB), focuses on biochemical physiology, primarily bioenergetics/energy metabolism, cell biology, cellular stress responses, enzymology, intermediary metabolism, macromolecular structure and function, gene regulation, evolutionary genetics. Most studies focus on biochemical or molecular analyses that have clear ramifications for physiological processes.
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