Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-07-09 DOI:10.1038/s41467-024-50097-3

Weinan Sun, Linjie Xia, Jinwu Deng, Simin Sun, Dandan Yue, Jiaqi You, Maojun Wang, Shuangxia Jin, Longfu Zhu, Keith Lindsey, Xianlong Zhang, Xiyan Yang

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Abstract

The occurrence of whole-genome duplication or polyploidy may promote plant adaptability to harsh environments. Here, we clarify the evolutionary relationship of eight GhCIPK6 homologous genes in upland cotton (Gossypium hirsutum). Gene expression and interaction analyses indicate that GhCIPK6 homologous genes show significant functional changes after polyploidy. Among these, GhCIPK6D1 and GhCIPK6D3 are significantly up-regulated by drought stress. Functional studies reveal that high GhCIPK6D1 expression promotes cotton drought sensitivity, while GhCIPK6D3 expression promotes drought tolerance, indicating clear functional differentiation. Genetic and biochemical analyses confirm the synergistic negative and positive regulation of cotton drought resistance through GhCBL1A1-GhCIPK6D1 and GhCBL2A1-GhCIPK6D3, respectively, to regulate stomatal movement by controlling the directional flow of K⁺ in guard cells. These results reveal differentiated roles of GhCIPK6 homologous genes in response to drought stress in upland cotton following polyploidy. The work provides a different perspective for exploring the functionalization and subfunctionalization of duplicated genes in response to polyploidization.

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CIPK6同源基因在调控棉花抗旱性中的进化和亚功能化。

全基因组复制或多倍体的出现可能会促进植物对恶劣环境的适应性。在此，我们阐明了陆地棉（Gossypium hirsutum）中 8 个 GhCIPK6 同源基因的进化关系。基因表达和相互作用分析表明，GhCIPK6 同源基因在多倍体后发生了显著的功能变化。其中，GhCIPK6D1 和 GhCIPK6D3 在干旱胁迫下显著上调。功能研究发现，GhCIPK6D1的高表达促进了棉花对干旱的敏感性，而GhCIPK6D3的表达则促进了棉花对干旱的耐受性，显示出明显的功能分化。遗传和生化分析证实，GhCBL1A1-GhCIPK6D1和GhCBL2A1-GhCIPK6D3分别通过控制K+在保卫细胞中的定向流动来调节气孔运动，从而协同负向和正向调节棉花的抗旱性。这些结果揭示了GhCIPK6同源基因在陆地棉多倍体后应对干旱胁迫中的不同作用。这项工作为探索多倍体化过程中重复基因的功能化和亚功能化提供了一个不同的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.