Understanding the role of P-type ATPases in regulating pollen fertility and development in pigeonpea.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rishu Jain, Harsha Srivastava, Kuldeep Kumar, Sandhya Sharma, Anandita Singh, Kishor Gaikwad
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Abstract

The P-type ATPase superfamily genes are the cation and phospholipid pumps that transport ions across the membranes by hydrolyzing ATP. They are involved in a diverse range of functions, including fundamental cellular events that occur during the growth of plants, especially in the reproductive organs. The present work has been undertaken to understand and characterize the P-type ATPases in the pigeonpea genome and their potential role in anther development and pollen fertility. A total of 59 P-type ATPases were predicted in the pigeonpea genome. The phylogenetic analysis classified the ATPases into five subfamilies: eleven P1B, eighteen P2A/B, fourteen P3A, fifteen P4, and one P5. Twenty-three pairs of P-type ATPases were tandemly duplicated, resulting in their expansion in the pigeonpea genome during evolution. The orthologs of the reported anther development-related genes were searched in the pigeonpea genome, and the expression profiling studies of specific genes via qRT-PCR in the pre- and post-meiotic anther stages of AKCMS11A (male sterile), AKCMS11B (maintainer) and AKPR303 (fertility restorer) lines of pigeonpea was done. Compared to the restorer and maintainer lines, the down-regulation of CcP-typeATPase22 in the post-meiotic anthers of the male sterile line might have played a role in pollen sterility. Furthermore, the strong expression of CcP-typeATPase2 in the post-meiotic anthers of restorer line and CcP-typeATPase46, CcP-typeATPase51, and CcP-typeATPase52 in the maintainer lines, respectively, compared to the male sterile line, clearly indicates their potential role in developing male reproductive organs in pigeonpea.

Abstract Image

了解 P 型 ATP 酶在调节鸽子豆花粉育性和发育中的作用。
P 型 ATP 酶超家族基因是阳离子和磷脂泵,通过水解 ATP 跨膜运输离子。它们参与了多种功能,包括植物生长过程中发生的基本细胞事件,尤其是在生殖器官中。本研究旨在了解和鉴定鸽子豆基因组中的 P 型 ATP 酶及其在花药发育和花粉育性中的潜在作用。鸽子豆基因组中共预测出 59 个 P 型 ATP 酶。系统进化分析将这些ATP酶分为五个亚家族:11个P1B、18个P2A/B、14个P3A、15个P4和1个P5。23 对 P 型 ATP 酶被串联重复,导致它们在鸽子豆基因组中的进化过程中不断扩大。在鸽子豆基因组中搜索了所报道的花药发育相关基因的直向同源物,并通过 qRT-PCR 对特定基因在鸽子豆 AKCMS11A(雄性不育系)、AKCMS11B(保持系)和 AKPR303(生育力恢复系)花药减数分裂前期和后期的表达谱进行了研究。与恢复系和保持系相比,雄性不育系减数分裂后花药中 CcP-typeATPase22 的下调可能是造成花粉不育的原因之一。此外,与雄性不育系相比,CcP-typeATPase2 在雄性不育系恢复系的花药后期以及 CcP-typeATPase46、CcP-typeATPase51 和 CcP-typeATPase52 在雄性不育系的花药后期均有较强的表达,这清楚地表明了它们在鸽子豆雄性生殖器官发育中的潜在作用。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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