Metabolic picture of microbial interaction: chemical crosstalk during co-cultivation between three dominant genera of bacteria and fungi in medicinal plants rhizosphere.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Metabolomics Pub Date : 2024-07-09 DOI:10.1007/s11306-024-02138-7

Moustafa M Zohair, Wang Dongmei, Kuniyoshi Shimizu

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Abstract

Introduction: Microbial communities affect several aspects of the earth's ecosystem through their metabolic interaction. The dynamics of this interaction emerge from complex multilevel networks of crosstalk. Elucidation of this interaction could help us to maintain the balance for a sustainable future.

Objectives: To investigate the chemical language among highly abundant microbial genera in the rhizospheres of medicinal plants based on the metabolomic analysis at the interaction level.

Methods: Coculturing experiments involving three microbial species: Aspergillus (A), Trichoderma (T), and Bacillus (B), representing fungi (A, T) and bacteria (B), respectively. These experiments encompassed various interaction levels, including dual cultures (AB, AT, TB) and triple cultures (ATB). Metabolic profiling by LC-QTOFMS revealed the effect of interaction level on the productivity and diversity of microbial specialized metabolites.

Results: The ATB interaction had the richest profile, while the bacterial profile in the monoculture condition had the lowest. Two native compounds of the Aspergillus genus, aspergillic acid and the dipeptide asperopiperazine B, exhibited decreased levels in the presence of the AT interaction and were undetectable in the presence of bacteria during the interaction. Trichodermarin N and Trichodermatide D isolated from Trichoderma species exclusively detected during coexistence with bacteria (TB and ATB). These findings indicate that the presence of Bacillus activates cryptic biosynthetic gene clusters in Trichoderma. The antibacterial activity of mixed culture extracts was stronger than that of the monoculture extracts. The TB extract exhibited strong antifungal activity compared to the monoculture extract and other mixed culture treatments.

Conclusion: The elucidation of medicinal plant microbiome interaction chemistry and its effect on the environment will also be of great interest in the context of medicinal plant health Additionally, it sheds light on the content of bioactive constituents, and facilitating the discovery of novel antimicrobials.

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微生物相互作用的代谢图景：药用植物根瘤菌圈中三个优势菌属与真菌共培养过程中的化学串扰。

引言微生物群落通过新陈代谢相互作用影响着地球生态系统的多个方面。这种相互作用的动态源于复杂的多级串联网络。阐明这种相互作用有助于我们保持平衡，实现可持续发展的未来：基于相互作用水平的代谢组学分析，研究药用植物根瘤中大量微生物属之间的化学语言：方法：涉及三种微生物物种的共培养实验：方法：涉及三种微生物物种的共培养实验：曲霉（A）、毛霉（T）和芽孢杆菌（B），分别代表真菌（A、T）和细菌（B）。这些实验涵盖了不同的相互作用水平，包括双重培养（AB、AT、TB）和三重培养（ATB）。利用 LC-QTOFMS 进行的代谢分析表明了相互作用水平对微生物特化代谢产物的产量和多样性的影响：结果：ATB 相互作用产生了最丰富的代谢谱，而单一培养条件下的细菌代谢谱最低。曲霉属的两种本地化合物--曲霉酸和二肽曲霉哌嗪 B--在 AT 相互作用时含量下降，而在细菌作用时则检测不到。从毛霉中分离出的毛霉素 N 和毛霉苷 D 只在与细菌（TB 和 ATB）共存时才被检测到。这些发现表明，芽孢杆菌的存在激活了毛霉中的隐性生物合成基因簇。混合培养提取物的抗菌活性强于单一培养提取物。与单培养提取物和其他混合培养处理相比，TB 提取物表现出较强的抗真菌活性：此外，它还揭示了生物活性成分的含量，有助于发现新型抗菌药物。

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来源期刊

Metabolomics 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.80%

发文量

审稿时长

2 months

期刊介绍： Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.