Defining blood hematology reference values in female pig-tailed macaques (Macaca nemestrina) using the Isolation Forest algorithm

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Medical Primatology Pub Date : 2024-07-08 DOI:10.1111/jmp.12723

Daniel Kim, Abigail Derton, George Khalil, Yi Pan, Shanon Bachman, Kristen Kelley, Gerardo Garcίa-Lerma, Charles W. Dobard, Michele B. Daly

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引用次数: 0

Abstract

Background

Pig-tailed macaques (PTMs) are commonly used as preclinical models to assess antiretroviral drugs for HIV prevention research. Drug toxicities and disease pathologies are often preceded by changes in blood hematology. To better assess the safety profile of pharmaceuticals, we defined normal ranges of hematological values in PTMs using an Isolation Forest (iForest) algorithm.

Methods

Eighteen female PTMs were evaluated. Blood was collected 1–24 times per animal for a total of 159 samples. Complete blood counts were performed, and iForest was used to analyze the hematology data to detect outliers.

Results

Median, IQR, and ranges were calculated for 13 hematology parameters. From all samples, 22 outliers were detected. These outliers were excluded from the reference index.

Conclusions

Using iForest, we defined a normal range for hematology parameters in female PTMs. This reference index can be a valuable tool for future studies evaluating drug toxicities in PTMs.

Abstract Image

查看原文本刊更多论文

使用隔离森林算法确定雌性猪尾猕猴（Macaca nemestrina）的血液参考值。

背景：猪尾猕猴（PTMs）通常被用作临床前模型，以评估用于艾滋病预防研究的抗逆转录病毒药物。在出现药物毒性和疾病病理变化之前，血液学往往会发生变化。为了更好地评估药物的安全性，我们使用隔离森林（iForest）算法定义了 PTMs 血液学值的正常范围：方法：评估了 18 只雌性 PTM。方法：对 18 只雌性 PTM 进行了评估，每只动物采血 1-24 次，共采集 159 个样本。进行了全血细胞计数，并使用 iForest 分析血液学数据以检测异常值：结果：计算了 13 个血液学参数的中位数、IQR 和范围。从所有样本中检测出 22 个异常值。这些异常值被排除在参考指数之外：利用 iForest，我们定义了女性 PTM 血液学参数的正常范围。结论：我们利用 iForest 定义了女性 PTM 血液学参数的正常范围，该参考指数可作为未来评估 PTM 药物毒性研究的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.