Schisandrin A can promote the anti-tumor effect of 5-Fu by reversing the immunosuppressive state of the body in rat.

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2024-07-09 DOI:10.1080/10286020.2024.2375291

Xiao-Hui Wang, Peng-Fei Dong, Feng Wang, Lin Zhou

引用次数: 0

Abstract

In this study, based on Walker 256 in vitro experiments, CCK-8 assay, clone formation assay, wound healing assay, and flow cytometry were used to detect cell apoptosis and cell cycle. It was found that schisandrin may have significant anti-tumor effects in vitro by inhibiting TGF-β/Smad signaling pathway. In addition, in vivo experiments, immunohistochemistry was used to observe the expression of HIF-1α, VEGF and VEGFR-2 in tumor tissues. It was found that schisandrin could significantly improve the immunosuppression induced by 5-Fu and enhance the antitumor effect of 5-Fu. The mechanism may be related to the inhibition of Wnt-1/β-catenin signaling pathway.

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五味子甲素可以通过逆转大鼠体内的免疫抑制状态来促进 5-Fu 的抗肿瘤作用。

本研究以 Walker 256 体外实验为基础，采用 CCK-8 试验、克隆形成试验、伤口愈合试验和流式细胞术检测细胞凋亡和细胞周期。结果发现，五味子甲素可通过抑制 TGF-β/Smad 信号通路在体外发挥显著的抗肿瘤作用。此外，在体内实验中，免疫组化法观察了肿瘤组织中 HIF-1α、VEGF 和 VEGFR-2 的表达。结果发现，五味子素能明显改善5-Fu诱导的免疫抑制，增强5-Fu的抗肿瘤作用。其机制可能与抑制Wnt-1/β-catenin信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.