Inhibition of Monilinia fructicola sporulation and pathogenicity through eucalyptol-mediated targeting of MfCat2 by Streptomyces lincolnensis strain JCP1-7.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Shan Chen, Haorong Yang, Meijun Chen, Weina Liu, Shaorui Tian, Rong Mu, Fan Jia, Changyun Liu, Guanhua Ma, Xianchao Sun, Guokang Chen
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引用次数: 0

Abstract

Peach brown rot, attributed to Monilinia fructicola, presents a significant threat to postharvest peach cultivation, causing losses of up to 80%. With an increasing number of countries, spearheaded by the European Union, imposing bans on chemical agents in fruit production, there is a growing interest in mining highly active antibacterial compounds from biological control strains for postharvest disease management. In this study, we highlight the unique ability of Streptomyces lincolnensis strain JCP1-7 to inhibit M. fructicola sporulation, despite its limited antimicrobial efficacy. Through GC-MS analysis, eucalyptol was identified as the key compound. Fumigation of diseased fruits with eucalyptol at a concentration of 0.0335 μg cm-3 demonstrated an in vivo inhibition rate against M. fructicola of 93.13%, completely suppressing spore formation. Transcriptome analysis revealed the impact of eucalyptol on multiple pathogenesis-related pathways, particularly through the inhibition of catalase 2 (Cat2) expression. Experiments with a MfCat2 knockout strain (ΔMfCat2) showed reduced pathogenicity and sensitivity to JCP1-7 and eucalyptol, suggesting MfCat2 as a potential target of JCP1-7 and eucalyptol against M. fructicola. Our findings elucidate that eucalyptol produced by S. lincolnensis JCP1-7 inhibits M. fructicola sporulation by regulating MfCat2, thereby effectively reducing postharvest peach brown rot occurrence. The use of fumigation of eucalyptol offers insights into peach brown rot management on a large scale, thus making a significant contribution to agricultural research.

林肯链霉菌 JCP1-7 株通过桉叶油醇介导的 MfCat2 靶向作用抑制果核莫尼菌孢子和致病性。
桃褐腐病是由果核菌(Monilinia fructicola)引起的,对桃采后栽培构成了严重威胁,造成的损失高达 80%。随着以欧盟为首的越来越多的国家禁止在水果生产中使用化学制剂,人们对从生物防治菌株中挖掘高活性抗菌化合物用于采后病害管理的兴趣与日俱增。在本研究中,我们强调了林肯链霉菌(Streptomyces lincolnensis)菌株 JCP1-7 抑制果蝇孢子的独特能力,尽管其抗菌效果有限。通过气相色谱-质谱分析,桉叶油醇被确定为关键化合物。用 0.0335 μg cm-3 浓度的桉叶油醇熏蒸病果,对果蝇孢子菌的体内抑制率为 93.13%,完全抑制了孢子的形成。转录组分析表明,桉叶油醇对多种致病相关途径都有影响,尤其是通过抑制过氧化氢酶 2(Cat2)的表达。用MfCat2基因敲除菌株(ΔMfCat2)进行的实验表明,该菌株的致病性降低,对JCP1-7和桉叶油醇的敏感性也降低,这表明MfCat2是JCP1-7和桉叶油醇防治果蝇的潜在靶标。我们的研究结果阐明,林肯菌 JCP1-7 产生的桉叶油醇可通过调节 MfCat2 抑制果腐菌孢子的生长,从而有效减少采后桃褐腐病的发生。使用桉叶油醇熏蒸为大规模管理桃褐腐病提供了启示,从而为农业研究做出了重大贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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