Evaluating the eDNA spatial signal in natural populations and its application for the monitoring of the threatened Harttiella (Siluriformes, Loricariidae)

IF 2.1 3区 地球科学 Q2 LIMNOLOGY
Céline Condachou, Opale Coutant, Sébastien Brosse, Jérôme Murienne
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Abstract

To face the current downward trajectory of freshwater biodiversity loss, the implementation of effective biodiversity monitoring programs is of utmost importance. Environmental DNA offers unprecedented opportunities for this aim but several challenges still need to be addressed before implementing efficient species monitoring using eDNA. One of them is optimizing the eDNA sampling scheme to maximize the eDNA detection probability. For instance, in flowing freshwaters, the transport of eDNA downstream from its source can impact the eDNA detection probability, and blur the link between eDNA detection and the local occurrence of the species. Here, we investigated the eDNA spatial range of Harttiella lucifer (Siluriformes, Loricariidae), a threatened neotropical siluriform fish inhabiting French Guianese mountain streams, and confined to waterfalls and fast-flowing environments. Environmental samples were collected at 11 sites from the H. lucifer population to 2000 m downstream. A species-specific dPCR approach was applied to quantify the amount of DNA present in each sample and evaluate the eDNA detection probability of H. lucifer according to the distance from its source. Results showed an accumulation of eDNA at 50 and 100 m downstream from H. lucifer population. The evaluation of detection probabilities revealed that 300 m downstream from H. lucifer population, the probability of detection drops to 50%. This study suggests that eDNA drift in neotropical small streams is limited to a few tens meters downstream. These findings demonstrate that in neotropical small streams, eDNA provides a picture of the local fish fauna rather than integrating information over large spatial scales.

Abstract Image

评估 eDNA 在自然种群中的空间信号及其在监测濒危哈氏蝠鲼中的应用
面对当前淡水生物多样性丧失的下降趋势,实施有效的生物多样性监测计划至关重要。环境 DNA 为实现这一目标提供了前所未有的机遇,但在利用 eDNA 实施有效的物种监测之前,仍需应对若干挑战。其中之一就是优化 eDNA 采样方案,最大限度地提高 eDNA 的检测概率。例如,在流动的淡水中,eDNA 从源头向下游的迁移会影响 eDNA 的检测概率,并模糊 eDNA 检测与物种在当地出现之间的联系。在此,我们研究了Harttiella lucifer(丝形目,Loricariidae)的eDNA空间分布范围,Harttiella lucifer(丝形目,Loricariidae)是一种濒危的新热带丝形鱼类,栖息在法属圭亚那的山溪中,仅限于瀑布和湍急的水流环境。从 H. lucifer 种群到下游 2000 米的 11 个地点采集了环境样本。应用物种特异性 dPCR 方法量化了每个样本中的 DNA 含量,并根据与 H. lucifer 来源的距离评估了 eDNA 的检测概率。结果显示,在距离 H. lucifer 种群下游 50 米和 100 米处,eDNA 有所积累。对检测概率的评估显示,在距离荧光虫种群下游300米处,检测概率下降到50%。这项研究表明,eDNA在新热带小溪流中的漂移范围仅限于下游几十米处。这些研究结果表明,在新热带小溪流中,eDNA提供的是当地鱼类动物群的情况,而不是大空间尺度的信息整合。
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来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
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